Fig. 1

cspC expression under different media conditions. Cultures of NA1000 harboring the cspC-lacZ transcriptional fusion were grown in the indicated media, and cspC expression was assessed by β-galactosidase activity assays. a cspC expression under nutrient starvation. Cultures grown in M2G to an OD₆₀₀ = 0.3 were washed and ressuspended in M2G (control), M2-G (without glucose) or M2–N (without ammonium chloride). b cspC expression in different types of media. Cultures were grown in the following media: M2 with 0.2 % glucose (M2G); M2 in which glucose was replaced by 0.2 % peptone (M2P); PYE and PYE containing 0.2 % glucose (PYEG). Beta-galactosidase activity assays were performed at time points: 0 h, 18 h, 24 h, and 48 h. Asterisks indicate a statistically significant difference in a two-way ANOVA test, with p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***). Statistical analysis of data indicated in (B) is relative to the same time point in M2G. Data are mean values of at least two independent experiments. Vertical black bars indicate the standard deviation