Fig. 10

The mapping profiles for IL24 in Replicate PFE1. In non-stranded RNA-seq, all reads mapped to IL24 are counted regardless if they are in the forward or reverse strands. However, in stranded RNA-seq, nearly all reads are mapped to the “+” strand and thus not counted because these reads are not reverse complementary to IL24 in the “+” strand. However, the coverage pattern of sequence reads does not support the sequence reads mapped to the IL24 genomic region that truly originate from this gene. All genes, transcripts, and sequence reads are colored in blue if they are in the “+” strand and colored in green if in the “−“ strand