Application of whole genome re-sequencing data in the development of diagnostic DNA markers tightly linked to a disease-resistance locus for marker-assisted selection in lupin ( Lupinus angustifolius )

Table 6 Conversion of SNP markers identified from genotyping markers on genetic linkage map flanking the R gene PhtjR into sequence-specific PCR markers suitable for genotyping by high resolution melting (HRM) with LightScanner

Marker	Primers	Primer sequence (5′-3′)
DAFWA926	DAFWA926F	GGTTGGGTTAACTTTTATGTCTAAAATC
DAFWA926	DAFWA926R	GGTAAGTTTATTTTTCTAAAGTTGAAC
DAFWA2836	DAFWA2836F	CACATAAGAATATGGAAATGGAGA
DAFWA2836	DAFWA2836R	CTGTAAACTGAAGGTGGGCATT
DAFWA3794	DAFWA3794F	GAAAGGAGAAAACTAATCAACATAAG
DAFWA3794	DAFWA3794R	ATTAGGGTTTGAGATAGAGTAACAT
DAFWA2747	DAFWA2747F	CCTAACTTCCGATCCAGTAAGC
DAFWA2747	DAFWA2747R	CTTTGATCGCTTGGGTTTC
DAFWA6277	DAFWA6277F	TTCGGGAATTTGTATGAGCT
DAFWA6277	DAFWA6277R	GGATGGATTCAAAGGTTCAAG
DAFWA8077	DAFWA8077F	GAGATTATTTTCACAAGCTTCCTC
DAFWA8077	DAFWA8077R	CCTTTTAGCTTATTCAATTAGCTTG
DAFWA6895	DAFWA6895F	TGAAGGTCCAATACCAGCAAG
DAFWA6895	DAFWA6895R	CAACTTCCCTGGAGCAAAA
DAFWA4020	DAFWA4020F	CTAGATAGTTTCGTTTTATCATAC
DAFWA4020	DAFWA4020R	GACATAAAGCTTATATATTTGCA
DAFWA3123	DAFWA3123F	CCCTGGACTCTCTCCCTGTATT
DAFWA3123	DAFWA3123R	GAATGAAAGTTTGATATGCATAATAA
DAFWA4021	DAFWA4021F	GCTCAGAAACGGTGTCGTT
DAFWA4021	DAFWA4021R	GAAGACCTCCAAAACCAAAGC

ISSN: 1471-2164