| |
Roots
|
Shoots
|
|---|
|
− N
|
+ Gln
|
+ Gln/ − N (fold change)
|
− N
|
+ Gln
|
+ Gln/ − N (fold change)
|
|---|
|
Glu
|
550.9 ± 52.9
|
669.3 ± 83.8
|
1.22 ± 0.17
|
1243.9 ± 280.7
|
1126.5 ± 205.5
|
0.93 ± 0.21
|
|
Asp
|
252.7 ± 39.0
|
350.8 ± 51.8
|
1.40 ± 0.21*
|
281.0 ± 60.3
|
228.0 ± 66.0
|
0.82 ± 0.18
|
|
Ser
|
171.7 ± 22.3
|
181.4 ± 17.2
|
1.06 ± 0.10
|
874.7 ± 224.9
|
666.0 ± 164.1
|
0.77 ± 0.16
|
|
Gln
|
108.1 ± 15.0
|
1275.5 ± 397.8
|
11.62 ± 2.36*
|
622.9 ± 302.7
|
469.0 ± 262.3
|
0.75 ± 0.21
|
|
Ala
|
81.1 ± 11.7
|
142.9 ± 24.7
|
1.76 ± 0.21*
|
1063.5 ± 297.9
|
847.3 ± 183.3
|
0.81 ± 0.11
|
|
Val
|
46.5 ± 6.8
|
47.9 ± 5.5
|
1.04 ± 0.15
|
225.7 ± 64.7
|
160.4 ± 38.3
|
0.73 ± 0.12
|
|
Cys
|
44.9 ± 11.0
|
46.8 ± 4.7
|
1.08 ± 0.19
|
340.7 ± 96.2
|
273.1 ± 81.3
|
0.80 ± 0.07
|
|
Leu
|
36.9 ± 6.9
|
35.2 ± 4.4
|
0.98 ± 0.22
|
60.4 ± 13.0
|
44.1 ± 9.0
|
0.74 ± 0.08
|
|
Thr
|
36.2 ± 2.1
|
39.1 ± 4.1
|
1.08 ± 0.13
|
160.0 ± 44.3
|
120.9 ± 34.7
|
0.76 ± 0.13
|
|
Pro
|
30.8 ± 13.5
|
28.4 ± 9.2
|
0.99 ± 0.30
|
166.4 ± 83.1
|
139.4 ± 79.8
|
0.82 ± 0.09
|
|
Ile
|
28.1 ± 4.5
|
25.5 ± 2.9
|
0.93 ± 0.17
|
49.8 ± 17.9
|
32.4 ± 7.2
|
0.68 ± 0.12
|
|
Trp
|
26.8 ± 9.6
|
26.5 ± 9.6
|
0.99 ± 0.06
|
416.7 ± 286.2
|
297.7 ± 144.1
|
0.78 ± 0.14
|
|
Lys
|
26.7 ± 5.1
|
27.4 ± 3.6
|
1.05 ± 0.19
|
183.0 ± 144.5
|
136.1 ± 95.7
|
0.80 ± 0.10
|
|
Tyr
|
24.9 ± 4.5
|
29.1 ± 2.0
|
1.19 ± 0.15
|
333.9 ± 87.0
|
264.9 ± 72.7
|
0.81 ± 0.19
|
|
His
|
24.0 ± 10.6
|
20.5 ± 5.6
|
0.91 ± 0.17
|
609.1 ± 488.3
|
396.3 ± 289.7
|
0.68 ± 0.14
|
|
Asn
|
21.6 ± 11.4
|
21.5 ± 11.2
|
1.03 ± 0.40
|
130.7 ± 95.3
|
119.9 ± 84.8
|
0.96 ± 0.32
|
|
Arg
|
20.2 ± 4.3
|
14.8 ± 3.5
|
0.74 ± 0.14
|
60.9 ± 16.6
|
43.6 ± 11.4
|
0.72 ± 0.12
|
|
Phe
|
14.8 ± 2.4
|
15.7 ± 3.6
|
1.06 ± 0.16
|
378.6 ± 96.2
|
291.5 ± 61.8
|
0.78 ± 0.09
|
|
Gly
|
14.6 ± 1.2
|
14.1 ± 1.9
|
0.96 ± 0.07
|
87.6 ± 25.3
|
67.6 ± 17.8
|
0.80 ± 0.21
|
|
Met
|
9.0 ± 0.7
|
10.4 ± 1.0
|
1.16 ± 0.11
|
60.4 ± 13.1
|
44.5 ± 7.5
|
0.74 ± 0.05
|
- − N without nitrogen, + Gln treated with 2.5 mM glutamine for 30 min. Results were derived from four biological replicates. *indicates p < 0.05 after analysis with Student’s t-test
