Fig. 7

qRT-PCR validation of differentially expressed genes. a Expression of 10 genes was validated using qRT-PCR and compared with their expression obtained from RNA-seq. qRT-PCR was performed and values were normalized relative to the expression levels of 18S rRNA in the same cDNA sample. Data are the means (± SE) of three replicates Expression data are presented as expression values of genes in stigma sample relative to their expression in flower sample (b) correlation of gene expression results obtained from qPCR and RNA-seq