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Table 2 Loci encoding well-characterized peptide products, which contain SNPs between the Serengeti-transformed stock and reference Muguga strain. Variants were also found in 47 hypothetical proteins (Additional file 2: Table S2)

From: The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence

Locus Tag /2005

Locus Tag IDa

Product Name

TM Domainsb

Protein Localization / Confidencec

Serengeti-transformed SNPsd

Kiambu5 SNPse

Syn

Nsyn

Syn

Nsyn

TP01_0011

TpMuguga_01g00011f

ABC transporter

11

_ / 2

9

11

≥66f

≥14f

TP02_0016

TpMuguga_02g00016f

ABC transporter

13

_ / 2

2

2

≥21f

≥7f

TP02_0017

TpMuguga_02g00017f

ABC transporter

12

_ / 3

19

17

≥11f

≥8f

TP02_0940

TpMuguga_02g00940f

ABC transporter

12

_ / 1

1

 

≥0f

≥0f

TP02_0951

TpMuguga_02g00951f

ABC transporter

11

_ / 1

44

37

≥9f

≥2f

TP03_0007

TpMuguga_03g00007f

ABC transporter

11

_ / 1

7

4

≥11f

≥9f

TP03_0864

TpMuguga_03g00864

ABC transporter

13

_ / 2

5

3

10

6

TP04_0021

TpMuguga_04g00021f

ABC transporter

13

_ / 1

10

24

≥43f

≥23f

TP02_0449

TpMuguga_02g00449

DNA replication factor CDT1 like

0

_ / 2

4

  

1

TP02_0448

TpMuguga_02g00448

F-box-like/WD repeat-containing protein TBL1XR1

0

_ / 1

 

1

 

1

TP03_0280

TpMuguga_03g00280

Papain family cysteine protease

1

_ / 2

12

7

1

1

TP04_0278

TpMuguga_04g00278

Translation initiation factor IF-2

1

S / 2

4

8

 

5

TP04_0279

TpMuguga_04g00279

Translation initiation factor IF-2

1

S / 2

1

3

  

TP04_0280

TpMuguga_04g00280f

Translation initiation factor IF-2

1

S / 1

1

3

≥2f

≥2f

  1. aLocus Tag Identifiers according to the updated whole genome gene structural re-annotation
  2. bNumber of transmembrane domains
  3. cProtein localization predictions based on TargetP: C = chloroplast; M = mitochondrion; S = Secretory pathway; _ = Any other location. Prediction confidence: 1 = max; 5 = min
  4. dSNPs between the Serengeti-transformed isolate and the reference Muguga isolate. Both synonymous (Syn) and non-synonymous (Nsyn) SNP counts are shown
  5. eSNPs between Kiambu5 and the reference Muguga isolate
  6. fThis genes coding sequence(s) had at least one position with <10x mapping coverage in the Kiambu5 stock, and thus there is likely more variation in the actual sequence