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Fig. 2 | BMC Genomics

Fig. 2

From: Using mixtures of biological samples as process controls for RNA-sequencing experiments

Fig. 2

Distributions of empirical (light) and ERCC-estimated (dark) enrichment ratios between SEQC samples A:B, A:C, and A:D. The empirical distribution was simulated from a normal distribution with means of 2.87 and 2.003 and standard deviations of 0.095 and 0.124 for samples A and B, as reported previously [37]. The ERCC-estimated values were calculated from Equation 3. Individual labs’ RNA enrichment varied inside a narrow range, yielding discrete peaks in the distribution for some outlying labs

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