Fig. 1

Microscopic visualization of brown rust (P. triticina) spore development and micronecrotic reaction of plant cells adjacent to haustorium mother cell (HMC) in TcLr9. Leaves of 7-day-old seedlings were inoculated with brown rust spores. Leaf samples, collected 4, 8, 11, 16, 20, 24, 28, 32, 36 and 44 h post inoculation (hpi), were stained with calcofluor white and analyzed under a fluorescence microscope. Representative images are shown with germinating spore, 4 hpi (a), appressorium on the guard cells, 4 hpi (b), haustorium mother cell, 16 hpi (c), necrotic cells formed near haustorium mother cell, 28 hpi (d). Indicated are: spore of brown rust (Sp), germ tube of a germinating spore (GT), appressorium (a), infection hypha (IH), haustorium mother cell (HMC), and necrotic cells (NC). Bar = 100 μm