Evolutionary divergence of core and post-translational circadian clock genes in the pitcher-plant mosquito, Wyeomyia smithii

Table 3 Sequencing results and assembly statistics

Sequencing results	Total	Aligned	Assembled
Number of reads^a	1,081,284	999,150	936,525
Number of bases	432,542,060	401,519,734	380,523,665
Assembly statistics	Singletons	Number of Contigs (Contigs >500 bp)	Isotigs
Number of sequences	54,418	25,904 (14,459)	21,233
Number of bases	20,109,580	19,120,348 (16,952,075)	32,183,643
Average sequence size (bp)	355	738 (1,172)	1,515
Length of N50 sequence (bp)^b	451	1,249 (1,373)	1,373

^aAfter quality filtering steps and removal of outliers such as adaptor sequences and repeats
^bN50 is a weighted median statistic, such that 50% of all bases are contained in sequences ≥N50 length

ISSN: 1471-2164