Fig. 3
The expression of genes involved in Nrf2 signaling and glutathione metabolism is altered in zebrafish exposed to DDVP. a DDVP exposure and constitutive Nrf2 activation affect similar sets of genes. The heat map compares the direction of change in liver message levels in DDVP-exposed zebrafish and KEAP-HKO mice with constitutively active Nrf2 relative to unexposed fish and normal mice. Mouse and functional data are taken from [46]. Zebrafish gene symbols are to the left, and mouse symbols are to the right. We show genes that could be definitively mapped between zebrafish and mice and that met our criteria for expression (see Methods). RefSeq and probe IDs are presented in Additional file 6. b The heat map depicts changes in the expression of genes involved in glutathione metabolism and precursor biosynthesis. In a few cases, the association between a gene and a database could not be definitively made. In these instances, tentative characterizations are shown in blue on the right and the database identifier is shown on the left. Changes in gene expression are shown as the log2 ratio relative to control. L, M, and H indicate low, mid, and high exposure concentrations