Fig. 5

Regulated splicing of bZIP68 caused by ER stress. a Predicted spliced sequence of the bZIP68 transcript. The arrows indicate the predicted cleavage sites, and the nucleotides in red are conserved in IRE1 substrates. b Loop structure at the predicted splicing site in bZIP68 mRNA. Each of the two loops contains three conserved nucleotides (marked in red) present in IRE1 substrates. c ER stress-induced splicing of bZIP68 mRNA. Total RNA was isolated from soybean seedlings that were untreated (lane 1) or treated with tunicamycin for 8 h (lane 2) and 24 h (lane 3) and used as a template for RT-PCR with a combination of primers for spliced and unspliced bZIP68