Fig. 5

RT-PCR validation of alternatively spliced transcripts of BPHLNC-unc241. Isoform-specific primers were designed for ten isoforms. Five of them were successfully amplified followed by sequencing. The PCR product size and the sequencing result of variant J was not as expect (a). The expressions of five isoforms in the third instar to fifth instar nymph and adult were measured, suggesting transcript variants vary in their expression profiles (b,c)