Table 2 Single nucleotide polymorphisms (SNPs) between TCH1516/FPR3757 and UAS391. Consensus base represents TCH1516 and FPR3757, allele represents a corresponding base in UAS391. Predicted amino acid changes in UAS391 compared to TCH1516 and FPR3757 were not displayed for synonymous SNPs

G

T

USA300HOU_0286/SAUSA300_0267

Leu1Phe

IS1272 transposase

T

C

USA300HOU_0155/SAUSA300_0145

Phosphate-import protein PhnD/ABC Transporter, periplasmic binding protein

G

A

USA300HOU_0521/SAUSA300_0513

Met386Ile

gtlX: glutamyl-tRNA synthetase

A

C

USA300HOU_1051/SAUSA300_1013

Asn267Thr/Asn263Thr

Bacterial cell division membrane protein FtsW

A

G

USA300HOU_1162/SAUSA300_1119

Glu524Gly

fakA: Fatty acid kinase

G

T

USA300HOU_1166/SAUSA300_1123

Gly169Val

fabD: malonyl CoA-acyl carrier protein transacylase (Lipid metabolism)

A

G

USA300HOU_1372/SAUSA300_1327

Val1114Ala

Ebh: Cell Wall-Associated Fibronectin-Binding Protein

C

A

USA300HOU_2197/SAUSA300_2161

Hyaluronate lyase is a glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.

G

A

USA300HOU_2631/SAUSA300_2566

Pro27Ser

HTH-type Transcriptional regulator ArcR, signal transduction system

C

T

USA300HOU_2641/SAUSA300_2576

PTS system, fructose-specific II ABC component, multi protein system involved in regulation of metabolic and transcriptional process

T

C

USA300HOU_2319/SAUSA300_2285

Aldose 1-epimerase

G

A

USA300HOU_2602/SAUSA300_2542

Arg517* ¹

Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases (Lipid metabolism)

A

C

USA300HOU_2654/SAUSA300_2589

Ser1782Ala

Serine-rich adhesin for platelets, cell surface protein precursor; KxYKxGKxW signal peptide

C

T

USA300HOU_0502/SAUSA300_0486

Thr85Met

Uncharacterized protein YabR

C

T

USA300HOU_1626/SAUSA300_1585

Ser35Leu

tRNA threonylcarbamoyladenosine dehydratase (Cells lacking this gene have a normal growth phenotype, but are unable to survive in a competitive growth situation with the wild-type strain. They display only the t6A but not the ct6A modification in tRNAs, and have lower decoding efficiency than wild-type. They show no defects in antibiotic sensitivity. In growth competition experiments, a tcdA mutant shows reduced fitness compared to wild-type, but outcompetes a csdA mutant.)

A

G

USA300HOU_2026/SAUSA300_1984

Lys118Arg

Putative membrane peptidase YdiL

T

C

USA300HOU_1488/SAUSA300_1436

phage lipoprotein

T

-

USA300HOU_1338/SAUSA300_1298

Phe130fs²

5-bromo-4-chloroindolyl phosphate hydrolysis protein