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Table 4 Bacterial strains and plasmids used in this study

From: Pangenome analysis of Bifidobacterium longum and site-directed mutagenesis through by-pass of restriction-modification systems

Strain/plasmid Relevant characteristics Additional Information Reference or source
E.coli strains    
EC101 Cloning host, repA+ kmr   [51]
EC101 pNZEM-M.blmncII Containing M.blmNCII Type II R-M MTase B8809_0607 This study
EC101 pNZEM -M.blmncIII Containing M.blmNCIII Type II R-M MTase B8809_0958 This study
EC101 pWSK29-MS.blmncI Containing MS1.blmNCI Type I R-M MTase and HsdS1 B8809_1353 and B8809_1354 This study
EC101 pNZ44-S.blmncI Containing S2.blmNCI Type I R-M HsdS2 B8809_1352 This study
EC101 pWSK29-MSS.blmncI Containing MS1.blmNCI and p44 + S2.blmNCI Type I R-M MTase, HsdS1 and HsdS2 B8809_1353, B8809_1354 and B8809_1352 This study
B.longum strains    
NCIMB 8809 Isolated from nursling stool   
CCUG 30698 Isolated from adult intestine   
NCIMB 8809-ArfB pORI19-tetMod-ArfB insertion mutant of NCIMB 8809 579 bp internal fragment of arfB and tetMod gene This study
Plasmids    
pAM5 pBC1 –puC19-Tcr   [70]
pPKCM7 pblueCm harbouring rep pCIBA089   [71]
pNZ44 Cmr, expression vector   [72]
pNZ44-S.blncI pNZ44 derivative containing blncIS   This study
pWSK29    [73]
pNZ8048-Em Emr; nisin-inducible translational fusion vector   [74]
pNZEM -M.blmncII pNZEM derivative containing M.blmNCII Type II R-M MTase B8809_0607 This study
pNZEM -M.blmncIII pNZEM derivative containing M.blmNCIII Type II R-M MTase B8809_0958 This study
pWSK29-MS.blmncI pWSK29 derivative containing MS1.blmNCI Type I R-M MTase and HsdS1 B8809_1353 and B8809_1354 This study
pNZ44-S.blmncI pNZ44 derivative containing S2.blmNCI Type I R-M HsdS2 B8809_1352 This study
pWSK29-MSS.blmncI pWSK29 derivative containing MS1.blmNCI and p44 + S2.blmNCI Type I R-M MTase, HsdS1 and HsdS2 B8809_1353, B8809_1354 and B8809_1352 This study
pORI19 Emr, repA-, ori+, cloning vector   [51]
pORI19-tet-ArfB Internal 579 bp fragment of arfB and tetW cloned into pORI19   This study