Table 4 Bacterial strains and plasmids used in this study

E.coli strains

EC101

Cloning host, repA⁺ km^r

[51]

EC101 pNZEM-M.blmncII

Containing M.blmNCII

Type II R-M MTase B8809_0607

This study

EC101 pNZEM -M.blmncIII

Containing M.blmNCIII

Type II R-M MTase B8809_0958

This study

EC101 pWSK29-MS.blmncI

Containing MS1.blmNCI

Type I R-M MTase and HsdS1 B8809_1353 and B8809_1354

This study

EC101 pNZ44-S.blmncI

Containing S2.blmNCI

Type I R-M HsdS2 B8809_1352

This study

EC101 pWSK29-MSS.blmncI

Containing MS1.blmNCI and p44 + S2.blmNCI

Type I R-M MTase, HsdS1 and HsdS2 B8809_1353, B8809_1354 and B8809_1352

This study

B.longum strains

NCIMB 8809

Isolated from nursling stool

CCUG 30698

Isolated from adult intestine

NCIMB 8809-ArfB

pORI19-tetMod-ArfB insertion mutant of NCIMB 8809

579 bp internal fragment of arfB and tetMod gene

This study

Plasmids

pAM5

pBC1 –puC19-Tc^r

[70]

pPKCM7

pblueCm harbouring rep pCIBA089

[71]

pNZ44

Cm^r, expression vector

[72]

pNZ44-S.blncI

pNZ44 derivative containing blncIS

This study

pWSK29

[73]

pNZ8048-Em

Emr; nisin-inducible translational fusion vector

[74]

pNZEM -M.blmncII

pNZEM derivative containing M.blmNCII

Type II R-M MTase B8809_0607

This study

pNZEM -M.blmncIII

pNZEM derivative containing M.blmNCIII

Type II R-M MTase B8809_0958

This study

pWSK29-MS.blmncI

pWSK29 derivative containing MS1.blmNCI

Type I R-M MTase and HsdS1 B8809_1353 and B8809_1354

This study

pNZ44-S.blmncI

pNZ44 derivative containing S2.blmNCI

Type I R-M HsdS2 B8809_1352

This study

pWSK29-MSS.blmncI

pWSK29 derivative containing MS1.blmNCI and p44 + S2.blmNCI

Type I R-M MTase, HsdS1 and HsdS2 B8809_1353, B8809_1354 and B8809_1352

This study

pORI19

Emr, repA-, ori+, cloning vector

[51]

pORI19-tet-ArfB

Internal 579 bp fragment of arfB and tetW cloned into pORI19

This study