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Fig. 4 | BMC Genomics

Fig. 4

From: Identification of ISMyo2, a novel insertion sequence element of IS21 family and its diagnostic potential for detection of Mycobacterium yongonense

Fig. 4

Specificity test for the real-time PCR assay developed for the identification of M. yongonense with using 28 reference strains of Mycobacterium species. M. yongonense was specifically identified based on Cq and melting temperature measurements. The tested strains are the same as those listed in Additional file 5 and were tested in duplicate via SYBR Green I real-time PCR. a, amplification curves; b, melting curve analysis

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