Fig. 2

Identification of genes expressed in tissues of interest and validation of microarray results. Representative examples from the WISH screen. a Blastema expression. The lower half of the head of the animal on the right in each pair was removed two to four days before fixation, on the day of greatest expression change for each gene. Arrows indicate the blastema. b Volcano plot of p-value vs. fold change for blastema to control comparison colored to indicate genes with no increased expression in the blastema (red), moderate blastema expression (green; e.g., F-box and leucine rich repeat 4) or strong blastema expression (blue; e.g., monocarboxylate transporter). c Neoblast expression. The animals on the right were irradiated (60 Gy) 3 days before staining to destroy the neoblasts. The animals on the left are non-irradiated. d Volcano plot colored to indicate neoblast expression; green marks genes detected in neoblasts and red marks non-neoblast genes. Genes were scored as expressed in neoblasts if staining was reduced following irradiation, as for histone h2a and jerky homolog-like. e CNS expression. Arrows and arrowheads indicate expression in the cephalic ganglia and ventral nerve cords, respectively. f Volcano plot colored to indicate genes with no CNS expression (red), weak CNS expression (green; e.g., Rb-like protein) or strong CNS expression (blue; e.g., prohormone-4, neurensin-1, and thioredoxin). g Venn diagram showing overlap of expression between tissues of interest. h Examples of genes not expressed in the blastema, neoblasts, or CNS, but in the epidermis (fam166b protein), intestine (Contig3907_SE3), secretory cells (Contig7601_SE3), or other discrete cells (mannosyl phosphorylinositol ceramide synthase sur1). i Examples of WISH to genes with proposed opposite side of the head expression. The lower half of the head of the animal on the right in each pair was amputated 2 to 4 days before fixation. The arrow indicates cephalic ganglia expression. Animals were imaged ventrally, anterior to the left. Scale = 0.5 mm