Fig. 5

Shows an alignment of MHC class I amino acid sequences predicted from selected BAC sequences published by Gao et al., 2010 in comparison with selected class I loci from the sheep reference chromosome 20 (NCBI accession NC_019477.1) and six class I reference sequences from the IPD database (identified as N*xxxxx) plus an alternative transcript sequence AAA31566. Nomenclature for the predicted genes from BAC sequences derived as follows: NCBI accession number, plus ‘rc’ if the sequence was reverse complemented before analysis, plus the class I gene identifier within the BAC. Sheep reference sequences identified by gene symbol in the NCBI Gene database. IPD sequences identified according to IPD allele name in the IPD-MHC database (http://www.ebi.ac.uk/cgi-bin/ipd/mhc/view_nomenclature.cgi?ovar.n). AAA31566 identified as an allele of Locus 8 (_Loc8) by Ballinghall, et al. 2008. Vertical lines delineate functional domains. A vertical box surrounds a motif in the transmembrane region important in classification of class I sequences as classical or nonclassical in cattle and sheep