Fig. 1

Categorisation of transcription start sites. Panel a: Initial TSS categorisation into primary (pTSS), secondary (sTSS), internal sense (isTSS) and internal antisense (asTSS) based on differential RNA sequencing. Panel b: Comparison of dRNA-Seq and gRNA-Seq data for genes BCAL0038-0040, visualised with the Integrated Genome Browser [51]. dRNA-Seq data are represented as read starts per base, with a vertical scale of 100 for forward reads and 7000 for reverse reads. gRNA-Seq data are represented as coverage, with a vertical scale of 500 for forward reads and 1000 for reverse reads. Panel c: Re-categorisation of internal TSS guided by leading edges of transcription identified by global RNA sequencing