Fig. 3

Phylogenetic trees depicting the Betaproteobacteria class and the Rhodocyclales, the only DPRB-containing order. a The alignment consisted of 21601 non-redundant amino acid positions from 147 orthologous genes. The tree was calculated using the LG substitution matrix and overlaying the results of 100 bootstrap replicates over the most likely tree of 50 independent inferences. b The Rhodocyclales subtree is rooted to taxa from a sister clade within the same order, which were chosen based on their position in the tree made for all Betaproteobacteria (i.e. Fig. 5A). The alignment used to construct this tree consisted of 139587 amino acid positions from 229 genes across 19 taxa. The tree was calculated under the LG + F substitution matrix and was generated by drawing 100 bootstrap replicates on the best tree chosen from 50 independent maximum-likelihood inferences. For both trees, all nodes had 100 % bootstrap support and the scale bar indicates the branch lench corresponding to 0.1 substitutions per site. DPRB are bolded and in green