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Fig. 2 | BMC Genomics

Fig. 2

From: A newly discovered Bordetella species carries a transcriptionally active CRISPR-Cas with a small Cas9 endonuclease

Fig. 2

Eleven additional B. pseudohinzii isolates possess the CRISPR-cas locus. PCR amplicons of cas9 (a), cas1 (b), cas2 (c) and the CRISPR array (d) from 12 B. pseudohinzii isolates. Lane 1: isolate 8-296-03; lanes 2–12: sequentially obtained isolates #2 to #12, respectively (Additional file 1: Table S1); Lane 13: negative control PCR. e Schematic representation of the CRISPR array in each of the isolates. Squares represent spacer sequences. Diamonds represent direct repeats. Arrows in panel D denote isolates that are missing spacer sequences Sp14 (isolate #2), Sp8 to Sp11 (#6) and Sp9 (#10) in panel e

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BMC Genomics

ISSN: 1471-2164

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