Fig. 9

Phylogenetic tree of the NPC2 gene family in Anopheles species. Aedes aegypti Niemann-Pick C2 proteins from four phylogentic groups (AAEL009557, AAEL001650, AAEL004120, AAEL006854) were used in BLASTp to identify the orthologues in Anopheles gambiae s.l. Anopheles christyi and An. epiroticus were included as outgroups. BLASTp returned a total of 108 orthologues in Anopheles species, separated into four groups based on the Ae. Aegypti orthologue. BLASTp and phylogenetic analysis of AAEL009557 returned forty proteins in six subgroups (group 1, blue font), AAEL001650 returned thirty proteins in five subgroups (group 2, gold font), AAEL004120 returned nine proteins in one subgroup (group 3, black font), and AAEL006854 returned twenty-nine proteins in four subgroups (group 4, pink font) The genome location of An. gambiae s.s orthologues are listed after each gene. Groups with >50 % bootstrap support are red. An. gambiae orthologues appear in a larger font with the IVM and blood meal-responsive transcripts in the largest font. Note the duplication appearing in the same physical location in group 4a