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Table 3 EST-SSRs tested empirically in two Eudicotyledoneae genera, Trifolium and Centaurea

From: Mining microsatellite markers from public expressed sequence tags databases for the study of threatened plants

Locus GenBank accession No. Species Primer sequences Repeat motif Expected size (bp) N A Size range (bp)
Forward Reverse
T6-Trifolium gi86106666 T. pratense CAACCAGTGGTGTGAGTAGGAG ACGTTGGTGGAGAGGTTGAG (AG)11 110–128 2 114–116
T7-Trifolium gi428283538 T. repens ATCACGCTTCACTCCTCCAC CAACTCCAAGCTTAAGATCGTGTA (AG)13 110–122   no PCR product
T1-Trifolium gi428292074 T. repens AGATTCCCACCAATCTCCCT CAATACGCGGGTCTTGATCT (AG)11 210–228 --- 257–261
T2-Trifolium gi86106666 T. pratense TTCCGGTTAGGTTAGGGTTT TTTTCACATCTTCCGAAGCC (AAT)7 110–113   no PCR product
T3-Trifolium gi428285635 T. repens CACCACATATGCAACCACAA GTCGACGACGGTTGTTACCT (AGT)8 110–126   no PCR product
T8-Trifolium gi428291122 T. repens GCAAAACTCAAGAGAACGGC GGATGTCTTCGGAGGTGAGA (ACC)7 110–122   no PCR product
T9-Trifolium gi428292435 T. repens ACAACCCATTTGCCTCAAAG TTTTCACTTCCACCACCTCC (ACC)7 110–133 2 124–127
T10-Trifolium gi86119186 T. pratense TCCACTAGTTCTAGAGCGGC TCCTGTAAACTGGAGGAGCC (ACC)9 110–153   no PCR product
T11-Trifolium gi86124411 T. pratense TGGCGGTGGTGACTTATACA TGTTTGGCAGTGGTGATGTT (AGG)8 110–153   no PCR product
T4-Trifolium gi86125686 T. pratense GCTGCCACAGCACTACCAG AATATTACCGTGAATGAAGCTCAG (ACC)8 110–113 1 110
T5-Trifolium gi86097190 T. pratense TGAGTTCCGAGTTAAGGCTCA TTCGGTAACTCCGAGGATTG (ACCT)5 210–217 2 227–230
T12-Trifolium gi428282514 T. repens GATTATTCAACCAAACGCCG TAGAAAGCCACGCCAAGACT (AATCC)20 290   no PCR product
C6-Centaurea gi124618051 C. maculosa TGGGATGCAGTCCAGTCATA TTGCAACTTGCCTGTACCAC (AC)11 160–162 1 256
C1-Centaurea gi148298213 C. maculosa GGGAACCACACCTTTCATCT GATCTGGCTTGACCCAAGAA (AC)10 90–119 2 99–101
C7-Centaurea gi124669731
C2-Centaurea gi124680442 C. solstitialis CGCATTATGGAATAAACCCG GCTTTCGACTTCATAAGCGG (AAG)7 140–152 1 147
C8-Centaurea gi148296795 C. maculosa CGATGTATACAGGTGGTGCG GGAGAAGGGGAGACGTAAGG (ACC)7 110–150 2 141–144
C9-Centaurea gi124675484 C. solstitialis AACGGTAGGAACCAGCATTG GATCCTCTGGCAGGGTCATA (ACC)9 260–302 4 290–299
C10-Centaurea gi124661102 C. solstitialis AGTTGCCAGAAAGGAGCAAG TCGAGAACAATGGCCTATCC (AGC)7 210–229   no PCR product
C11-Centaurea gi148292432 C. maculosa TCCATGGATACAACCACCAA GCGATATTCGGATGCAAAGT (AGG)7 160–175 4 160–172
C3-Centaurea gi124632630 C. maculosa GCCATCCCCTTCTCTACTCC GTTACAGGTGACGATGGGG (AGT)7 160–181   no PCR product
C4-Centaurea gi124691992 C. solstitialis CTGCACCTACCCAGAGAAGC CGGGAGAGGGTAAATTGTGA (AGGT)5 110–115 3 103–109
C12-Centaurea gi124632477 C. maculosa ATGCATTGAGAAGGCCAATC AACTCGCAAGCCTTTTCAAG (AATCGG)4 210–223   no PCR product
C5-Centaurea gi124673348 C. solstitialis TTAAGCATTCTTCGAGGCGT TCTATGCCTACGCCGATCTC (AAGCAG)5 110   no PCR product
  1. GenBank accession No., identification number of the EST sequences (when more than one ID refers to consensus sequence); species, indicates the species of the EST sequences; primer sequences; type of repeated motif; expected size of the PCR product; N A, number of alleles for the examined individuals and size range of the PCR product (−− indicates stutter peak)