In silico hybridization enables transcriptomic illumination of the nature and evolution of Myxozoa

BMC Genomics

Table 1 Sequencing, assembly, and in silico hybridization statistics

Assembly
Raw reads	409,520,180
Reads after quality control	195,155,200
Assembled contigs	930,419
Contigs after redundancy filtration	575,740
Length min-max	200–16,127
Average length	802
N50	1288
G-C content	33.30 %
Raw reads mapped to contigs	193,101,058 (47.2 %)
In silico pipeline
Only matched to Myxozoa	232,396
Stronger match to Myxozoa	39,651
Total identified as myxozoan	272,047 (47.3 %)
Only matched to host	15,057
Stronger match to host	85,043
Match to other contamination	335
Total identified as host/contamination	100,435 (17.4 %)
Too close to call^a	14,084
No significant match found	189,174
Total without annotation	203,258 (35.3 %)

For in silico pipeline statistics, percentages in parentheses reflect proportion of contigs assigned to that category out of the redundancy-filtered set of contigs
^aMatched both myxozoan and host query sets with e-values within two orders of magnitude of one another

ISSN: 1471-2164