Fig. 2

Correlation of DHA drug-induced and developmental transcriptional changes from RNA-seq data. RNA-seq data from untreated P. falciparum K1 ring (a), trophozoite (b) and schizont (c) synchronized parasites were compared with published RNA-seq data from time-points taken during the intra-erythrocytic developmental cycle (IDC) (Bártfai et al. dataset [58], red triangles; Otto et al. dataset [57], blue squares; Siegel et al. dataset [59], open circles). Pearson correlation coefficients (PCC) were calculated from 3839 genes expressed in all time-points, defined as hours post-invasion (hpi). Transcriptomic changes in expression induced by 1 h DHA treatment for ring (d), trophozoite (e) and schizont (f) parasites were correlated with changes during the IDC (data and symbols as in parts A-C). The overlap of genes with significant change in expression under DHA treatment in ring and trophozoite parasites is shown for down-regulated genes in (g) and up-regulated genes in (h)