Fig. 1
From: Defining the nitrogen regulated transcriptome of Mycobacterium smegmatis using continuous culture
Growth of M. smegmatis in continuous culture under nitrogen-depleted and nitrogen-replete conditions. Optical density (OD600), residual glycerol and ammonium concentrations in the medium were monitored during the experiment. a The effect of a pulse with 25Â mM NH4Cl on residual ammonium (open squares) and glycerol (closed triangles) concentrations in the medium and OD600 (closed circles) in a nitrogen-depleted culture. A pulse with 10Â mM glycerol had no effect in a nitrogen-depleted culture. b The effect of a pulse with 30Â mM glycerol on residual glycerol (open triangles) and ammonium (closed squares) concentrations in the medium and OD600 (closed circles) in a nitrogen-replete culture. A pulse with 1Â mM NH4Cl had no effect on the nitrogen-replete culture, respectively