Fig. 1From: Whole genome sequencing reveals genomic heterogeneity and antibiotic purification in Mycobacterium tuberculosis isolatesValidation of variants identified by Illumina sequencing. Analysis of Sanger sequencing alignments and corresponding chromatograms was used to validate the presence of homo- and heterogeneous variants identified by Illumina sequencing. Variants present in either the sequencing file or in the chromatogram (Additional file 2: Table S2) were scored as true variants while sequences which remained wild-type were scored as falseBack to article page