Table 5 Isolate specific variants identified in rifampicin mono-resistant and MDR M. tuberculosis isolates of patient 1 and 2
| Â | Isolate | Locus | Gene | Amino acid change | Coverage of variant (%) | Gene description | Functional categoryC |
|---|---|---|---|---|---|---|---|
Patient 1 | R721a | Rv0435c | Â | I397L | 50 | Putative conserved ATPase | Cell wall and cell processes |
Rv0435c | Â | D395Y | 50 | Putative conserved ATPase | Â | ||
Rv0668 | rpoC | V1039A | 30 | DNA-directed RNA polymerase RpoC (RNA polymerase beta’ subunit). | Information pathways | ||
Rv1850 | ureC | Q11K | 48 | Urease alpha subunit UreC (urea amidohydrolase) | Intermediary metabolism and respiration | ||
Rv1850 | ureC | Q11R | 49 | Urease alpha subunit UreC (urea amidohydrolase) | Â | ||
Rv3218 | Â | Y174H | 66 | Conserved protein | Conserved hypotheticals | ||
Rv2004c | Â | Ins AAG | 43 | Conserved protein | Conserved hypotheticals | ||
Rv3563 | fadE32 | Ins AC | 40 | Probable acyl-CoA dehydrogenase FadE32 | Lipid metabolism | ||
Rv3696c | glpK | Ins AC | 73 | Probable glycerol kinase GlpK (ATP:glycerol 3-phosphotransferase) | Intermediary metabolism and respiration | ||
R807b | Rv1908c | katG | G309V | 100 | Catalase-peroxidase-peroxynitritase T KatG | Virulence, detoxification, adaptation | |
Rv3696c | glpK | T91I | 80 | Probable glycerol kinase GlpK (glycerokinase) (GK) | Intermediary metabolism and respiration | ||
Patient 2 | R912a | Rv1128c | Â | G430S | 98 | Conserved hypothetical protein | Insertion sequences and phages |
Rv2236c | cobD | L269S | 97 | Probable cobalamin biosynthesis transmembrane protein CobD | Intermediary metabolism and respiration | ||
Rv2664 | Â | H22Q | 99 | Hypothetical protein | Conserved hypotheticals | ||
Rv2772c | Â | E149* | 97 | Probable conserved transmembrane protein | Cell wall and cell processes | ||
Rv2984 | ppk1 | P631A | 96 | Polyphosphate kinase PPK (polyphosphoric acid kinase) | Intermediary metabolism and respiration | ||
Rv3391 | acrA1 | syn (248) | 99 | Possible multi-functional enzyme with acyl-CoA-reductase activity AcrA1 | Lipid metabolism | ||
Rv3537 | kstD | syn (378) | 98 | Probable dehydrogenase | Intermediary metabolism and respiration | ||
R1210b |  | inhA promoter | −15 | 45 |  |  | |
Rv1484 | inhA | S94A | 70 | NADH-dependent enoyl-[acyl-carrier-protein] reductase InhA (NADH-dependent enoyl-ACP reductase) | Lipid metabolism | ||
Rv1629 | polA | syn (146) | 45 | Probable DNA polymerase I PolA | Information pathways | ||
Rv2935 | ppsE | C582R | 69 | Phenolpthiocerol synthesis type-I polyketide synthase PpsE | Lipid metabolism |