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Table 3 DGE sequencing statistics

From: Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis

Summary Control 0 h Exposure to Sulfide
6 h 24 h 48 h
Raw data Total number 4,089,259 3,772,420 4,214,487 4,218,738
Distinct tag number 223,037 194,769 183,978 196,142
Clean tags Total number 3,909,160 3,613,737 4,057,279 4,027,932
Distinct tag number 94,189 86,979 82,866 82,991
All tags mapping to a gene Total number 3,381,053 2,982,514 3,406,833 3,372,408
Total % of clean tags 86.49 % 82.53 % 83.97 % 83.73 %
Distinct tag number 60,344 49,296 49,136 47,831
Distinct tag % of clean tags 64.07 % 56.68 % 59.30 % 57.63 %
Unambiguous tags mapping to a gene Total number 2,843,143 2,484,170 2,865,778 2,839,135
Total % of clean tag 72.73 % 68.74 % 70.63 % 70.49 %
Distinct tag number 48,405 39,557 39,685 38,365
Distinct tag % of clean tags 51.39 % 45.48 % 47.89 % 46.23 %
All tag-mapped genes Number 21,121 20,526 20,129 20,111
% of reference genes 40.54 % 39.40 % 38.64 % 38.61 %
Unambiguous tag-mapped genes Number 14,722 14,264 13,937 13,832
% of reference genes 28.26 % 27.38 % 26.75 % 26.55 %
Unknown tags Total number 502,708 591,656 601,170 601,065
Total % of clean tags 12.86 % 16.37 % 14.82 % 14.92 %
Distinct tag number 32,399 35,742 31,937 33,194
Distinct tag % of clean tags 34.40 % 41.09 % 38.54 % 40.00 %
  1. The raw data are tags obtained from sequencing without processing. The clean tags are tags that remained after filtering out dirty (low-quality) tags from the raw data. The distinct tags are tags that do not overlap. The unambiguous tags are the clean tags that remained after the removal of tags mapped to reference sequences from multiple genes