Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis

BMC Genomics

Table 3 DGE sequencing statistics

Summary		Control 0 h	Exposure to Sulfide
Summary		Control 0 h	6 h	24 h	48 h
Raw data	Total number	4,089,259	3,772,420	4,214,487	4,218,738
Raw data	Distinct tag number	223,037	194,769	183,978	196,142
Clean tags	Total number	3,909,160	3,613,737	4,057,279	4,027,932
Clean tags	Distinct tag number	94,189	86,979	82,866	82,991
All tags mapping to a gene	Total number	3,381,053	2,982,514	3,406,833	3,372,408
	Total % of clean tags	86.49 %	82.53 %	83.97 %	83.73 %
	Distinct tag number	60,344	49,296	49,136	47,831
	Distinct tag % of clean tags	64.07 %	56.68 %	59.30 %	57.63 %
Unambiguous tags mapping to a gene	Total number	2,843,143	2,484,170	2,865,778	2,839,135
	Total % of clean tag	72.73 %	68.74 %	70.63 %	70.49 %
	Distinct tag number	48,405	39,557	39,685	38,365
	Distinct tag % of clean tags	51.39 %	45.48 %	47.89 %	46.23 %
All tag-mapped genes	Number	21,121	20,526	20,129	20,111
All tag-mapped genes	% of reference genes	40.54 %	39.40 %	38.64 %	38.61 %
Unambiguous tag-mapped genes	Number	14,722	14,264	13,937	13,832
Unambiguous tag-mapped genes	% of reference genes	28.26 %	27.38 %	26.75 %	26.55 %
Unknown tags	Total number	502,708	591,656	601,170	601,065
	Total % of clean tags	12.86 %	16.37 %	14.82 %	14.92 %
	Distinct tag number	32,399	35,742	31,937	33,194
	Distinct tag % of clean tags	34.40 %	41.09 %	38.54 %	40.00 %

The raw data are tags obtained from sequencing without processing. The clean tags are tags that remained after filtering out dirty (low-quality) tags from the raw data. The distinct tags are tags that do not overlap. The unambiguous tags are the clean tags that remained after the removal of tags mapped to reference sequences from multiple genes

ISSN: 1471-2164