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Table 1 Metrics describing the transcriptome alignments

From: Molecular tools for studying the major malaria vector Anopheles funestus: improving the utility of the genome using a comparative poly(A) and Ribo-Zero RNAseq analysis

Sample ID

Reads to align (R1 + R2)

Aligned reads (%)a

Aligned R1 (%)b

Aligned R2 (%)b

Aligned in pair (%)b

Properly paired (%)b,c

Singleton (%)b

F1_XP_RZ

67,895,442

37,480,868 (55 %)

20,409,963 (54 %)

17,070,905 (46 %)

28,766,542 (77 %)

26,655,176 (71 %)

8,714,326 (23 %)

F1_XP_PA

65,605,318

38,878,773 (59 %)

21,731,643 (56 %)

17,147,130 (44 %)

29,122,168 (75 %)

27,271,540 (70 %)

9,756,605 (25 %)

F1_RZ

60,479,632

21,002,025 (35 %)

11,311,755 (54 %)

9,690,270 (46 %)

15,677,600 (75 %)

14,192,682 (68 %)

5,324,425 (25 %)

F1_PA

63,555,450

37,671,216 (59 %)

20,881,499 (55 %)

16,789,717 (45 %)

28,690,598 (76 %)

26,499,330 (70 %)

8,980,618 (24 %)

F2_RZ

43,733,908

24,067,157 (55 %)

13,121,886 (55 %)

10,945,271 (45 %)

18,560,162 (77 %)

17,136,240 (71 %)

5,506,995 (23 %)

F2_PA

60,656,692

35,610,912 (59 %)

19,661,565 (55 %)

15,949,347 (45 %)

27,281,408 (77 %)

25,371,888 (71 %)

8,329,504 (23 %)

F3_RZ

54,693,906

25,673,135 (47 %)

13,831,002 (54 %)

11,842,133 (46 %)

19,086,154 (74 %)

17,431,254 (68 %)

6,586,981 (26 %)

F3_PA

60,211,066

35,007,336 (58 %)

19,424,414 (55 %)

15,582,922 (45 %)

26,507,306 (76 %)

24,701,646 (71 %)

8,500,030 (24 %)

  1. a% of reads to align
  2. b% of aligned reads
  3. cProperly paired means both read and its mate are mapped to opposing strands of the reference sequence, with 3′ ends innermost and within the allowed distance from each other (mean 0 bp, standard deviation 100 bp)