Fig. 2

Gonadal and histological structures of WT and dnd-MO gynogenetic fish during gonadal differentiation. Gonadal and histological structures of WT and dnd-MO gonads at 25 dph (a-f), 35 dph (g-l), 45 dph (m-r) and 60 dph (s-x). The external morphology of WT gonads (a, g, m and s), and dnd-MO gonads (b, h, n and t). Haematoxylin–eosin and immunofluorescence staining of WT gonads (c, d, i, j, o, p, u and v) and dnd-MO gonads (e, f, k, l, q, r, w and x). White arrow indicates PGC, arrow head indicates differentiating oogonia, star indicates primary oocyte and black arrow indicates cavity. [Scale bars, 500 mm (a, b, g and h), 1 cm (m, n, s and t), 20 μm (c and d) and others 50 μm