Fig. 5

MiR-4732-3p regulates TGF-β signaling and promotes erythroid cell proliferation. a Relative changes of TGF-β reporter activities in K562 cells co-transfected with luciferase constructs and microRNA mimics (n = 4) (**p < 0.01). Cells were transfected with microRNA mimic, SMAD4-responsive Firefly construct, and a separate Renilla construct as a transfection control. b Relative mRNA levels of p21, PAI-1, Bax, and Bim 24 h after transfection of K562s with microRNA mimics. Levels were determined using qPCR and normalized by GAPDH (n = 3) (*p < 0.05). c Total cell number of primary CD34 erythroid progenitors 48 and 72 h after equal numbers of cells were transfected with indicated microRNA mimics on day 8 of differentiation (n = 3) (*p < 0.05). d Total cell number of primary CD34 erythroid progenitors 24 h after equal numbers of cells were transfected with indicated combinations of microRNA mimics and overexpression constructs on day 8 of differentiation (n = 3) (*p < 0.05, **p < 0.01)