Fig. 2

Modifications of miRNA length expressed in the porcine endometria collected on different days of the estrous cycle or pregnancy. a Percent of modifications affecting either 3’ end, 5’ end or both ends; b Percent of additions and deletions on 3’ end, and percent of additions and deletions on 5’ end. c Mean values of normalized read counts from all groups for reference/canonical miR-140-3p or its isomiRs and sum of normalized read counts for each seed sequence. d Expression of reference/canonical miR-140-3p (seed sequence ACCACAG) and its 5’ isomiR with seed sequence CCACAGG. miRNA/isomiR expression on different days of the estrous cycle and pregnancy (n = 4-6 animals/group) is presented as mean of normalized reads counts (NRC) ± SEM. Two-way ANOVA analysis with Bonferoni post-hoc test was performed on log-transformed values to find statistical differences. Means with different superscripts differ significantly (small letters – the estrous cycle, capital letters – pregnancy). ** p < 0.01, indicate difference between reproductive states (estrous cycle vs. pregnancy) within the same day