Fig. 1

Translationally profiling in MCF7 and MCF10A cells. a Organisation of a mammalian mRNA. The m⁷GpppN 5′ cap is indicated as are potential RNA structural elements within the TL. The brown ovals indicate RNA binding proteins that modulate expression by binding to regions outside the principle ORF. The 3′ polyA tail (A)_n is also indicated. b Representative polysome profiles for MCF7 and MCF10A cells. Equivalent amounts of protein were loaded onto each gradient. Note that the polysomal fraction is considerably smaller in the non-tumoural MCF10A cell line. c Plot of the differential transcript levels (MCF7/MCF10A) in total versus polysomal RNA. The green dots indicate the up-regulated genes, the red dots the down-regulated genes. The Spearman correlation is indicated. d GO analysis of the translationally regulated genes from the RefSeq annotations. The GO pie charts, which represent the percentage of gene hits against the total number of genes, were built using the PANTHER (Protein ANalysis THrough Evolutionary Relationships) classification system [76]