Fig. 4

Identification of the hrpG regulon of strain CFBP 5828R of X. campestris pv. raphani by RNA sequencing. a Dot plot of the normalized expression levels of all genes in presence of hrpG* versus the empty vector (EV). The diagonal indicates genes which expression level is identical in both conditions. Red lines indicate boundaries where gene expression is induced five-fold relative to the other condition. Red dots indicate genes which expression is significantly induced at least five fold relative to the other condition. The hrpG gene is indicated in green. b Dot plot of the expression ratios (hrpG* versus EV) of 13 genes determined either by RNA sequencing (RNAseq) or quantitative RT-PCR (RT-qPCR). The diagonal indicates genes which expression ratios are identical with both techniques. Bars indicate standard deviations. c Box plot of the normalized expression ratios of hrpG-induced genes encoding the T3S system (T3SS) and type III secreted proteins (T3SP) versus the other genes (−). Box plot of the normalized expression ratios of hrpG-induced genes with a PIP box (TTCGB-N₁₅-TTCGB) in their promoter region versus the other genes (−). Red dots indicate the mean values. Significant mean difference was detected using a Welch-test (***, P-value < 0.001)