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Table 3 Differentially expressed novel miRNAs in shoot apexes between HT and HS broccoli under heat stress

From: Comparative miRNAs analysis of Two contrasting broccoli inbred lines with divergent head-forming capacity under temperature stress

miRNA

Library miRNA sequence (5'- > 3')

HT counta

HS counta

Fold change expression (HT/HS)

miRNA*

p-value

Signature (p < 0.001)

bol-novel-01

AGGAGUUAGGAUGAAGAAGCUAUC

123.42

25.33

4.62

N

0.00E + 00

TRUE

bol-novel-02

AGAGUGAAAAAUAGAGUGAUGAAC

59.73

15.01

3.72

N

3.07E-150

TRUE

bol-novel-03

UAGGAGUUAGGAUGAAGAAGCUA

41.82

8.19

4.77

N

1.29E-131

TRUE

bol-novel-04

AGGGAUAGUUGAGAAUGUGGCUGC

34.54

10.40

3.10

N

1.33E-71

TRUE

bol-novel-05

AAGGAGUGGAGUUUUGAAGAUAGA

48.58

21.25

2.13

N

4.84E-55

TRUE

bol-novel-06

GUGGAAGAAAGAGAAGAUGAUGUG

25.90

8.00

3.02

N

2.35E-52

TRUE

bol-novel-07

GGAGAUAGUUGGGAAUGUGGCUGC

29.66

12.46

2.22

N

4.38E-37

TRUE

bol-novel-09

GGGGCUGUAGAUGCUCUGGACAGC

87.33

213.99

0.38

N

0.00E + 00

TRUE

bol-novel-16

GUUGGCGUUAAGUGAAACGACGUC

9.35

48.49

0.18

N

1.49E-186

TRUE

bol-novel-17

AAGGCACGUGUCGUUGGCUAAG

21.46

68.26

0.29

N

2.83E-170

TRUE

bol-novel-18

GAGACGUUUGAGCUUCGACGC

12.30

45.21

0.25

N

7.55E-132

TRUE

bol-novel-23

GUAUUCUGACGGACAUUCCGACGG

30.44

63.54

0.45

N

6.36E-85

TRUE

bol-novel-25

CACGUGUCGUUGGCUAAGUCC

12.89

36.22

0.33

N

1.50E-78

TRUE

bol-novel-26

UGAAUUACUAUUGCGACGCGG

8.64

27.12

0.30

Y

1.29E-67

TRUE

bol-novel-27

CAGAAUCCGGGCUAGAAGCGA

25.42

50.85

0.47

Y

1.03E-62

TRUE

bol-novel-30

GGAUUGGCUCUGAGGGCUGGG

7.83

21.18

0.35

N

2.73E-44

TRUE

bol-novel-31

CGGAGGUAGGGUCCAGCGGCU

11.41

26.59

0.40

N

2.97E-44

TRUE

bol-novel-33

GAGACAGACUGUACUAGCGACGCU

11.49

25.79

0.42

N

2.50E-40

TRUE

bol-novel-34

AUUGGAUCUGAUUUCUUAAUCGGC

13.89

29.03

0.45

N

8.81E-40

TRUE

bol-novel-35

AGACAGACUGUACUAGCGACGC

12.34

26.74

0.43

N

2.40E-39

TRUE

bol-novel-36

ACUCGUGAUGGCGGACACCUCAGC

10.86

23.16

0.44

N

2.52E-33

TRUE

bol-novel-37

AUUUGGCACUGAUCAAUUGUUCGG

8.24

19.20

0.40

N

2.05E-32

TRUE

bol-novel-39

AUUAUUUGAACUUGGACUCAAUGG

11.86

23.04

0.48

N

1.49E-27

TRUE

bol-novel-40

ACCGUGGUGGUCCAGACUAGAGAC

8.64

16.61

0.49

N

8.45E-20

TRUE

  1. acount was normalized as transcripts per million (TPM)
  2. *miRNA* is from the stem–loop precursor miRNAs, which is processed into the miRNA/miRNA* duplex
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BMC Genomics

ISSN: 1471-2164

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