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Table 3 Agreement between qPCR and microarray data

From: Development and validation of a mixed-tissue oligonucleotide DNA microarray for Atlantic bluefin tuna, Thunnus thynnus (Linnaeus, 1758)

 

ALL DATA

UP

DOWN

Indice

non

TEF

non

TEF

non

TEF

Slope

0.91

0.99

0.88

1.05

0.82

1.05

Y intercept

−0.75

−0.24

−0.54

−0.59

−1.08

0.05

Precision (r)a

0.80

0.87

0.77

0.85

0.54

0.73

Accuracy

0.98

0.99

0.96

0.97

0.89

0.94

CCC

0.78

0.86

0.73

0.82

0.48

0.69

  1. Correlation correspondence index (CCC) and other indices describing agreement of microarray and qPCR data (non-normalised (non) and normalised (TEF)) are shown. Log2FCs (Fold Changes) were examined for all data and positively and negatively regulated values separately
  2. aCorrelation is significant at the 0.01 level (2-tailed)