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Table 1 High-throughput sequencing statistics of N. benthamiana sRNAs

From: Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis

   Redundant Non-redundant    Redundant Non-redundant
   Reads Matching N. benthamiana genome Reads Matching N. benthamiana genome    Reads Matching N. benthamiana genome Reads Matching N. benthamiana genome
Seedling 1 Raw reads 4 353 615     Stem 1 Raw reads 2 673 839    
  Adaptor removed 3 192 563   1 106 502    Adaptor removed 2 468 386   858 314  
  Filter by sequence properties 3 187 558   1 102 305    Filter by sequence properties 2 464 474   854 744  
  t/rRNA removed 3 034 009 2 791 655 1 085 271 942 175   t/rRNA removed 2 404 489 2 205 954 846 701 746 712
  Match known miRNAs 262 998 259 394 1 531 792   Match known miRNAs 121 361 119 819 1 376 860
Seedling 2 Raw reads 13 565 620     Stem 2 Raw reads 13 390 095    
  Adaptor removed 9 931 841   1 048 202    Adaptor removed 12 682 160   3 280 430  
  Filter by sequence properties 9 916 497   1 036 381    Filter by sequence properties 12 662 117   3 263 087  
  t/rRNA removed 9 461 135 8 656 790 1 016 371 841 123   t/rRNA removed 12 195 396 11 213 510 3 246 517 2 773 011
  Match known miRNAs 1 132 848 1 117 758 2 423 1 030   Match known miRNAs 664 082 654 984 3 455 1 969
Root 1 Raw reads 4 416 396     Flower 1 Raw reads 17 715 434    
  Adaptor removed 3 567 326   673 485    Adaptor removed 15 107 462   4 920 011  
  Filter by sequence properties 3 561 838   669 035    Filter by sequence properties 15 084 084   4 899 263  
  t/rRNA removed 3 250 883 2 926 826 652 495 553 372   t/rRNA removed 14 374 989 13 270 386 4 872 774 4 186 250
  Match known miRNAs 368 908 363 793 1557 827   Match known miRNAs 517 965 510 930 3 461 2 128
Root 2 Raw reads 6 227 488     Flower 2 Raw reads 8 784 135    
  Adaptor removed 5 472 237   2 020 365    Adaptor removed 8 156 033   2 71814  
  Filter by sequence properties 5 463 791   2 013 081    Filter by sequence properties 8 143 554   262 570  
  t/rRNA removed 5 041 719 4 673 608 1 992 006 1 746 022   t/rRNA removed 7 295 596 6 508 891 253 399 185 299
  Match known miRNAs 308 179 304 276 2 402 1 474   Match known miRNAs 817 620 807 317 1 526 547
Leaf 1 Raw reads 4 481 051     Leaf BTI Raw reads 2 646 118    
  Adaptor removed 3 752 733   1 675 147    Adaptor removed 2 373 816   445 460  
  Filter by sequence properties 3 746 943   1 670 236    Filter by sequence properties 2 370 062   442 372  
  t/rRNA removed 3 567 987 3 252 343 1 644 909 1 440 195   t/rRNA removed 2 277 189 2 092 901 433 297 375 749
  Match known miRNAs 258 610 255 179 2 368 1 502   Match known miRNAs 234 022 231 082 1 025 489
Leaf 2 Raw reads 3 801 050     Seedling BTI Raw reads 7 874 296    
  Adaptor removed 2 925 945   1 149 340    Adaptor removed 7 174 326   2 105 046  
  Filter by sequence properties 2 921 408   1 145 456    Filter by sequence properties 7 163 048   2 095 876  
  t/rRNA removed 2 834 354 2 596 026 1 131 710 982 432   t/rRNA removed 6 906 776 6 088 762 2 070 845 287 724
  Match known miRNAs 274 028 270 476 1 434 737   Match known miRNAs 786 266 423 581 2205 113