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Table 3 Targets of novel miRNAs found in Nicotiana benthamiana

From: Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis

new miRNA ID Gene ID Cleavage position Category Alignment score Normalized abundancea Annotation
Nb_miRC1_3p comp79937_c1_seq1 312_1058 862 3 3,5 0,2 Elongation factor 1-alpha 4
Nb_miRC1_3p comp78325_c0_seq3 335_2797 1128 1 1,0 0,2 Protein ROS1
Nb_miRC2_3p comp75577_c0_seq17 1347_1982 405 2 4,0 1,2 Down syndrome critical region protein 3 homolog
Nb_miRC3_5p comp79768_c0_seq4 287_3412 1868 2 3,5 1,1 Elongation factor Ts
Nb_miRC4_3p comp76033_c0_seq2 367_1701 917 2 4,0 1,9 CBL-interacting serine/threonine-protein kinase 5
Nb_miRC5_3p comp73317_c0_seq28 1673_2236 2967 0 3,5 2,1 Peroxisome biogenesis factor 10
Nb_miRC5_3p comp69461_c0_seq1 371_982 1380 2 0,5 0,2 Protein CREG1
Nb_miRC5_3p comp76292_c1_seq18 500_808 1632 2 1,5 0,2 Probable WRKY transcription factor 53
Nb_miRC5_3p comp72235_c0_seq2 239_1444 1720 2 2,5 0,3 Cytochrome b561 and DOMON domain-containing protein At5g47530-like
Nb_miRC5_5p comp70843_c0_seq1 178_1023 528 2 4,0 1,4 Protein TIC 21, chloroplastic
Nb_miRC6_3p comp75362_c2_seq1 7645_8472 2399 2 3,5 0,2 G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330
Nb_miRC7_3p comp73495_c0_seq6 312_1019 1288 2 3,5 1,4 Proteasome subunit alpha type-2-A
Nb_miRC7_3p comp56753_c0_seq1 215_457 517 0 2,0 5,5 WAT1-related protein At5g40240-like
Nb_miRC8_3p_a comp71383_c0_seq6 99_857 1317 3 2,5 0,6 Aquaporin TIP1-3
Nb_miRC8_3p_a comp75266_c2_seq4 3193_3486 3454 2 1,0 0,7 Probable isoaspartyl peptidase/L-asparaginase 3
Nb_miRC9_5p comp71496_c0_seq1 489_1463 462 2 4,0 0,2 Serine/threonine-protein phosphatase 2A regulatory subunit B” subunit alpha
Nb_miRC9_5p comp64986_c0_seq1 125_487 1232 2 2,5 0,2 60S ribosomal protein L34
Nb_miRC10_3p comp77607_c3_seq4 3_1151 2317 2 4,0 0,2 Suberization-associated anionic peroxidase
Nb_miRC10_3p comp80078_c3_seq4 193_2097 2131 2 1,0 1,1 Formate--tetrahydrofolate ligase
Nb_miRC10_3p comp72702_c0_seq4 152_2431 2575 3 2,0 1,0 Beta-amyrin synthase
Nb_miRC11_5p comp82078_c0_seq1 3_1277 938 2 3,5 2,7 Threonine deaminase
Nb_miRC11_5p comp78978_c0_seq3 374_2785 2557 2 3,0 0,4 Transcription factor RF2a/ Probable transcription factor PosF21
Nb_miRC11_5p comp89465_c0_seq1 218_1237 532 3 4,0 0,2 Ervatamin-B-like
Nb_miRC12_5p comp79295_c0_seq1 283_3321 3114 2 3,5 2,7 Probably inactive leucine-rich repeat receptor-like protein kinase At3g28040
Nb_miRC12_5p comp56752_c0_seq1 568 1 3,5 0,7 ZRT/IRT-like protein 1
Nb_miRC13_3p comp77835_c0_seq3 61_621 797 2 2,5 0,3 Disease resistance response protein 206
Nb_miRC14_5p comp74263_c0_seq1 230_2029 966 2 4,0 3,8 Chaperonin 60 subunit beta 2, chloroplastic
Nb_miRC15_5p comp78088_c1_seq1 1536_2366 3251 3 4,0 0,7 Peroxisome biogenesis protein 19-1
Nb_miRC15_5p comp85070_c0_seq1 3_2648 2151 2 2,5 1,0 (E,E)-geranyllinalool synthase
Nb_miRC16_3p comp88815_c0_seq1 2_1909 1845 2 2,5 0,9 Polyphenol oxidase, chloroplastic
Nb_miRC17_5p comp63365_c1_seq3 3_242 29 0 1,0 1,3 Heterotrimeric GTP binding protein alpha subunit
Nb_miRC18_3p comp67145_c0_seq1 106_930 361 2 3,5 2,0 50S ribosomal protein L3, chloroplastic
  1. a The abundance for the degradome fragment (tag) indicating cleavage at that position/total number of fragments x 1,000,000