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Table 3 Targets of novel miRNAs found in Nicotiana benthamiana

From: Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis

new miRNA ID

Gene ID

Cleavage position

Category

Alignment score

Normalized abundancea

Annotation

Nb_miRC1_3p

comp79937_c1_seq1 312_1058

862

3

3,5

0,2

Elongation factor 1-alpha 4

Nb_miRC1_3p

comp78325_c0_seq3 335_2797

1128

1

1,0

0,2

Protein ROS1

Nb_miRC2_3p

comp75577_c0_seq17 1347_1982

405

2

4,0

1,2

Down syndrome critical region protein 3 homolog

Nb_miRC3_5p

comp79768_c0_seq4 287_3412

1868

2

3,5

1,1

Elongation factor Ts

Nb_miRC4_3p

comp76033_c0_seq2 367_1701

917

2

4,0

1,9

CBL-interacting serine/threonine-protein kinase 5

Nb_miRC5_3p

comp73317_c0_seq28 1673_2236

2967

0

3,5

2,1

Peroxisome biogenesis factor 10

Nb_miRC5_3p

comp69461_c0_seq1 371_982

1380

2

0,5

0,2

Protein CREG1

Nb_miRC5_3p

comp76292_c1_seq18 500_808

1632

2

1,5

0,2

Probable WRKY transcription factor 53

Nb_miRC5_3p

comp72235_c0_seq2 239_1444

1720

2

2,5

0,3

Cytochrome b561 and DOMON domain-containing protein At5g47530-like

Nb_miRC5_5p

comp70843_c0_seq1 178_1023

528

2

4,0

1,4

Protein TIC 21, chloroplastic

Nb_miRC6_3p

comp75362_c2_seq1 7645_8472

2399

2

3,5

0,2

G-type lectin S-receptor-like serine/threonine-protein kinase At1g11330

Nb_miRC7_3p

comp73495_c0_seq6 312_1019

1288

2

3,5

1,4

Proteasome subunit alpha type-2-A

Nb_miRC7_3p

comp56753_c0_seq1 215_457

517

0

2,0

5,5

WAT1-related protein At5g40240-like

Nb_miRC8_3p_a

comp71383_c0_seq6 99_857

1317

3

2,5

0,6

Aquaporin TIP1-3

Nb_miRC8_3p_a

comp75266_c2_seq4 3193_3486

3454

2

1,0

0,7

Probable isoaspartyl peptidase/L-asparaginase 3

Nb_miRC9_5p

comp71496_c0_seq1 489_1463

462

2

4,0

0,2

Serine/threonine-protein phosphatase 2A regulatory subunit B” subunit alpha

Nb_miRC9_5p

comp64986_c0_seq1 125_487

1232

2

2,5

0,2

60S ribosomal protein L34

Nb_miRC10_3p

comp77607_c3_seq4 3_1151

2317

2

4,0

0,2

Suberization-associated anionic peroxidase

Nb_miRC10_3p

comp80078_c3_seq4 193_2097

2131

2

1,0

1,1

Formate--tetrahydrofolate ligase

Nb_miRC10_3p

comp72702_c0_seq4 152_2431

2575

3

2,0

1,0

Beta-amyrin synthase

Nb_miRC11_5p

comp82078_c0_seq1 3_1277

938

2

3,5

2,7

Threonine deaminase

Nb_miRC11_5p

comp78978_c0_seq3 374_2785

2557

2

3,0

0,4

Transcription factor RF2a/ Probable transcription factor PosF21

Nb_miRC11_5p

comp89465_c0_seq1 218_1237

532

3

4,0

0,2

Ervatamin-B-like

Nb_miRC12_5p

comp79295_c0_seq1 283_3321

3114

2

3,5

2,7

Probably inactive leucine-rich repeat receptor-like protein kinase At3g28040

Nb_miRC12_5p

comp56752_c0_seq1

568

1

3,5

0,7

ZRT/IRT-like protein 1

Nb_miRC13_3p

comp77835_c0_seq3 61_621

797

2

2,5

0,3

Disease resistance response protein 206

Nb_miRC14_5p

comp74263_c0_seq1 230_2029

966

2

4,0

3,8

Chaperonin 60 subunit beta 2, chloroplastic

Nb_miRC15_5p

comp78088_c1_seq1 1536_2366

3251

3

4,0

0,7

Peroxisome biogenesis protein 19-1

Nb_miRC15_5p

comp85070_c0_seq1 3_2648

2151

2

2,5

1,0

(E,E)-geranyllinalool synthase

Nb_miRC16_3p

comp88815_c0_seq1 2_1909

1845

2

2,5

0,9

Polyphenol oxidase, chloroplastic

Nb_miRC17_5p

comp63365_c1_seq3 3_242

29

0

1,0

1,3

Heterotrimeric GTP binding protein alpha subunit

Nb_miRC18_3p

comp67145_c0_seq1 106_930

361

2

3,5

2,0

50S ribosomal protein L3, chloroplastic

  1. a The abundance for the degradome fragment (tag) indicating cleavage at that position/total number of fragments x 1,000,000