Fig. 7From: Evidence of recent interspecies horizontal gene transfer regarding nucleopolyhedrovirus infection of Spodoptera frugiperda Transcription kinetics of SfMNPV ColA ORFs 023 and 024. Spodoptera frugiperda larvae were exposed to SfMNPV ColA and whole RNAs were isolated from sacrificed animals at different intervals post-infection. Then, cDNA with polyT primer was generated for each sample and PCR assays were done using specific primers which amplify fragments of different ORFs from SfMNPV ColA genome [polyhedrin (polh), immediate-early 0 (ie-0), ORF023 and ORF024]. Figure shows a photo cut-out showing the amplification bands resolved by agarose gel electrophoresis. The SfMNPV ColA genome was used as positive control and water was used as negative controlBack to article page