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Table 5 Summary of RT-PCR results for male-biased DEGs

From: Gonadal transcriptomic analysis and differentially expressed genes in the testis and ovary of the Pacific white shrimp (Litopenaeus vannamei)

Sequence ID Bioinformatic analysis Fold change Semi-quantitative PCR validation RQ (T/O) qPCR validation (T-Test) Annotation
Unigene916_All TB 9.65 / 18.28 ** --
Unigene13080_All TB 4.06 / 7.56 ** Solute carrier organic anion transporter family member 4C1
Unigene8661_All TB 11.95 / 97.07 ** --
Unigene1205_All TB 2.20 / 2.44 ** Neuron navigator 2
Unigene14488_All TB 8.11 / 58.95 ** --
Unigene8480_All TB 13.20 / 53.21 ** Transforming growth factor-beta-induced protein ig-h3
Unigene6291_All TB 46.10 / 64.34 ** Receptor-type tyrosine-protein phosphatase T
Unigene2551_All TB 11.69 / 59.28 ** Melanization interacting? protein
Unigene19396_All TB 24.32 / 200.74 ** --
Unigene2838_All TB 5.53 / 21.29 ** Intracellular fatty acid binding protein
CL911.Contig2_All TB 47.53 / 342.15 ** --
Unigene6879_All TB 56.75 / 84.84 ** Scylla paramamosain amino acids transporter
Unigene1920_All TB 34.02 / 186.80 ** PREDICTED: 4-coumarate--CoA ligase-like
Unigene6062_All TB 79.59 / 194.74 ** --
Unigene511_All TB 25.83 / 102.59 ** Beta,beta-carotene 15,15′-monooxygenase
Unigene15520_All TB 12.40 / 67.25 ** Cytochrome c oxidase subunit III
Unigene9139_All TB 11.49 / 196.53 ** NADH dehydrogenase subunit 4
Unigene5297_All TB 64.72 / 248.07 ** PREDICTED: metallothionein-1 F-like
Unigene6055_All TB 11.66 / 56.41 ** Neuroparsin 1 precursor
CL756.Contig1_All TB 16.05 / 19.52 ** Heme-binding protein 2
Unigene11959_All TB 948.78 / 1415.06 ** --
CL550.Contig2_All TB 27.87 / −5.60 ** Alpha-I tubulin
Unigene15830_All TB 825.49 / 3967.48 ** Histone H1-beta, late embryonic
Unigene7663_All TB 22.26 / 54.81 ** PREDICTED: DNA excision repair protein haywire
Unigene16117_All TB 4618.69 / 64216.17 ** Argonaute-3
CL2601.Contig2_All TB 11.15 / 28.15 ** Homo sapiens X BAC RP11-1051 N9
CL535.Contig2_All TB 133.51 / 33.55 ** Histone-lysine N-methyltransferase PRDM9
Unigene11824_All TB 9.23 / 12.89 ** Ankyrin repeat domain-containing protein 13B
CL2710.Contig1_All TB 10.35 / 13.38 ** Zinc finger protein on ecdysone puffs
Unigene3364_All TB 5.50 / 3.10 ** Iodide transporter-like protein
Unigene46_All TB 12.33 / 6.09 ** DNA ligase 4
CL730.Contig2_All TB 10.32 / 19.81 ** PREDICTED: HIG1 domain family member 1C
Unigene15329_All TB 18.22 / 21.07 ** Stromal antigen-like protein, copy A
Unigene15690_All TB 3.78 / 8994.17 ** --
Unigene15762_All TB 4.57 / 371865.46 ** --
Unigene20560_All TB 114.97 / 1014.87 ** --
CL951.Contig2_All TS / Not specific?? 947.72 ** --
CL1031.Contig1_All TS / Not specific 19779.35 ** Probable serine/threonine-protein kinase fhkB
CL273.Contig1_All TS / Not specific 39469.77 ** Eukaryotic translation initiation factor 4 gamma, putative
Unigene1966_All TS / Not specific 35.96 ** Tyrosine aminotransferase
Unigene16106_All TS / Not specific 148.83 ** --
Unigene23134_All TS / Specific / / --
Unigene29005_All TS / Specific / / Casein kinase I
Unigene29287_All TS / Specific / / DNA-directed RNA polymerase II subunit RPB2
Unigene28792_All TS / Specific / / --
Unigene28151_All TS / Specific / / Dual specificity testis-specific protein kinase 2
CL737.Contig2_All TS / Specific / / Penaeus monodon clone TUZX4-6:48 microsatellite sequence
  1. TB testis biased, TS testis specific, RQ relative expression level
  2. Asterisks indicate significant difference between ovary and testis expression of the gene by QPCR test ( *P<0.05, **P<0.01)