Table 5 Summary of RT-PCR results for male-biased DEGs
Sequence ID | Bioinformatic analysis | Fold change | Semi-quantitative PCR validation | RQ (T/O) | qPCR validation (T-Test) | Annotation |
|---|---|---|---|---|---|---|
Unigene916_All | TB | 9.65 | / | 18.28 | ** | -- |
Unigene13080_All | TB | 4.06 | / | 7.56 | ** | Solute carrier organic anion transporter family member 4C1 |
Unigene8661_All | TB | 11.95 | / | 97.07 | ** | -- |
Unigene1205_All | TB | 2.20 | / | 2.44 | ** | Neuron navigator 2 |
Unigene14488_All | TB | 8.11 | / | 58.95 | ** | -- |
Unigene8480_All | TB | 13.20 | / | 53.21 | ** | Transforming growth factor-beta-induced protein ig-h3 |
Unigene6291_All | TB | 46.10 | / | 64.34 | ** | Receptor-type tyrosine-protein phosphatase T |
Unigene2551_All | TB | 11.69 | / | 59.28 | ** | Melanization interacting? protein |
Unigene19396_All | TB | 24.32 | / | 200.74 | ** | -- |
Unigene2838_All | TB | 5.53 | / | 21.29 | ** | Intracellular fatty acid binding protein |
CL911.Contig2_All | TB | 47.53 | / | 342.15 | ** | -- |
Unigene6879_All | TB | 56.75 | / | 84.84 | ** | Scylla paramamosain amino acids transporter |
Unigene1920_All | TB | 34.02 | / | 186.80 | ** | PREDICTED: 4-coumarate--CoA ligase-like |
Unigene6062_All | TB | 79.59 | / | 194.74 | ** | -- |
Unigene511_All | TB | 25.83 | / | 102.59 | ** | Beta,beta-carotene 15,15′-monooxygenase |
Unigene15520_All | TB | 12.40 | / | 67.25 | ** | Cytochrome c oxidase subunit III |
Unigene9139_All | TB | 11.49 | / | 196.53 | ** | NADH dehydrogenase subunit 4 |
Unigene5297_All | TB | 64.72 | / | 248.07 | ** | PREDICTED: metallothionein-1Â F-like |
Unigene6055_All | TB | 11.66 | / | 56.41 | ** | Neuroparsin 1 precursor |
CL756.Contig1_All | TB | 16.05 | / | 19.52 | ** | Heme-binding protein 2 |
Unigene11959_All | TB | 948.78 | / | 1415.06 | ** | -- |
CL550.Contig2_All | TB | 27.87 | / | −5.60 | ** | Alpha-I tubulin |
Unigene15830_All | TB | 825.49 | / | 3967.48 | ** | Histone H1-beta, late embryonic |
Unigene7663_All | TB | 22.26 | / | 54.81 | ** | PREDICTED: DNA excision repair protein haywire |
Unigene16117_All | TB | 4618.69 | / | 64216.17 | ** | Argonaute-3 |
CL2601.Contig2_All | TB | 11.15 | / | 28.15 | ** | Homo sapiens X BAC RP11-1051Â N9 |
CL535.Contig2_All | TB | 133.51 | / | 33.55 | ** | Histone-lysine N-methyltransferase PRDM9 |
Unigene11824_All | TB | 9.23 | / | 12.89 | ** | Ankyrin repeat domain-containing protein 13B |
CL2710.Contig1_All | TB | 10.35 | / | 13.38 | ** | Zinc finger protein on ecdysone puffs |
Unigene3364_All | TB | 5.50 | / | 3.10 | ** | Iodide transporter-like protein |
Unigene46_All | TB | 12.33 | / | 6.09 | ** | DNA ligase 4 |
CL730.Contig2_All | TB | 10.32 | / | 19.81 | ** | PREDICTED: HIG1 domain family member 1C |
Unigene15329_All | TB | 18.22 | / | 21.07 | ** | Stromal antigen-like protein, copy A |
Unigene15690_All | TB | 3.78 | / | 8994.17 | ** | -- |
Unigene15762_All | TB | 4.57 | / | 371865.46 | ** | -- |
Unigene20560_All | TB | 114.97 | / | 1014.87 | ** | -- |
CL951.Contig2_All | TS | / | Not specific?? | 947.72 | ** | -- |
CL1031.Contig1_All | TS | / | Not specific | 19779.35 | ** | Probable serine/threonine-protein kinase fhkB |
CL273.Contig1_All | TS | / | Not specific | 39469.77 | ** | Eukaryotic translation initiation factor 4 gamma, putative |
Unigene1966_All | TS | / | Not specific | 35.96 | ** | Tyrosine aminotransferase |
Unigene16106_All | TS | / | Not specific | 148.83 | ** | -- |
Unigene23134_All | TS | / | Specific | / | / | -- |
Unigene29005_All | TS | / | Specific | / | / | Casein kinase I |
Unigene29287_All | TS | / | Specific | / | / | DNA-directed RNA polymerase II subunit RPB2 |
Unigene28792_All | TS | / | Specific | / | / | -- |
Unigene28151_All | TS | / | Specific | / | / | Dual specificity testis-specific protein kinase 2 |
CL737.Contig2_All | TS | / | Specific | / | / | Penaeus monodon clone TUZX4-6:48 microsatellite sequence |