Fig. 4

Phylogenetic tree of KS amino acid sequences from algal PKSs. From an alignment built by MUSCLE, a maximum likelihood (ML) tree was constructed by PhyML using sequences of FabF (involved in type II fatty acid biosynthesis) from cyanobacteria and algae as the outgroup. Branch support values were calculated with aBayes likelihood-based method. The scale bar indicates substitutions per site. Abbreviations: sKS, single (free-standing) KS domains; Ali, Aurantiochytrium limacinum; Ake, Aplanochytrium kerguelense; Aan, Aureococcus anophagefferens; Cpa, Cryptosporidium parvum; Cre, Chlamydomonas reinhardtii; Csu, Coccomyxa subellipsoidea; Cva, Chlorella variabilis; Cwa, Crocosphaera watsonii; Ehu, Emiliania huxleyi; Esi, Ectocarpus siliculosus; Fcy, Fragilariopsis cylindrus; Gvi, Gloeobacter violaceus; Mae, Microcystis aeruginosa; Mbr, Monosiga brevicolis; Mcc, Micromonas sp. CCMP1545; Mic, Micromonas sp. RCC299; Mne, Monoraphidium neglectum; Nga, Nannochloropsis gaditana; Noc, Nannochloropsis oceanica; Nos, Nostoc sp. PCC 7120; Npu, Nostoc punctiforme; Olu, Ostreococcus lucimarinus; Ota, Ostreococcus tauri; Pmu, Pseudo-nitzschia multiseries; Ptr, Phaeodactylum tricornutum; Sag, Schizochytrium aggregatum; Sch, Schizochytrium sp. ATCC 20888; Smi, Symbiodinium minutum; Tps, Thalassiosira pseudonana; Vca, Volvox carteri