Fig. 3
From: A human 3′UTR clone collection to study post-transcriptional gene regulation
The h3′UTRome v1 as a resource to detect miRNA targets in high throughput. 3′LIFE screen performed on 87 human 3′UTRs extracted from the h3′UTRome v1, queried against two miRNAs let-7c and miR-221. The repression index was calculated for each 3′UTR and indicates a normalized ratio between Firefly and Renilla luciferases. A minimum cutoff for repression was drawn at 0.8 and the asterisks denote statistically significant repression (p < 0.05). The top hits for each miRNA are displayed as a heat map on the right panels. Increased intensity of color indicates greater repression. 18 top hits were identified for let-7c of which 3 were previously validated (as denoted by the arrows). 13 top hits were identified for miR-221 of which 4 were previously validated (as denoted by the arrows)