Table 3 Hieratical analysis of molecular variance (AMOVA). The genetic diversity between species, among populations and among genotypes within populations of 51 genotypes of H. irregulare and H. annosum was shown. Loci localized more than 500 bp upstream from transcribed regions were referred to as OUT, loci positioned within 500–50 bp before 5’UTR were referred to as UP, loci inside exons were referred to as IN and loci within 50–500 bp after 3’UTR were referred to as DOWN. Degrees of freedom (d.f.) were also included
Source of variation | d.f. | Sum of square | Variance components | percentage variation (%) |
|---|---|---|---|---|
OUT | ||||
Between species | 1 | 71.086 | 1.27047 | 30.61 |
Among populations within species | 3 | 29.382 | 0.37090 | 8.94 |
Among genotypes within populations | 97 | 243.433 | 2.50962 | 60.46 |
Total | 101 | 343.901 | 4.15099 | 100.00 |
UP | ||||
Between species | 1 | 62.024 | 1.22320 | 42.53 |
Among populations within species | 3 | 10.512 | 0.09931 | 3.45 |
Among genotypes within populations | 97 | 150.690 | 1.55350 | 54.02 |
Total | 101 | 223.226 | 2.87602 | 100.00 |
IN | ||||
Between species | 1 | 25.901 | 0.51177 | 32.45 |
Among populations within species | 3 | 7.127 | 0.07491 | 4.75 |
Among genotypes within populations | 97 | 92.105 | 0.99038 | 62.80 |
Total | 101 | 125.133 | 1.57706 | 100.00 |
DOWN | ||||
Between species | 1 | 22.562 | 0.41370 | 24.74 |
Among populations within species | 3 | 8.064 | 0.07675 | 4.59 |
Among genotypes within populations | 97 | 114.521 | 1.18062 | 70.65 |
Total | 101 | 145.147 | 1.67107 | 100.00 |