Fig. 2

Growth curves of the best strains considering acetic acid tolerance obtained by transformation of S. cerevisiae BY4741_haa1Δ and BY4741 parental with ISA1307 genomic library plasmids. a S. cerevisiae BY4741_haa1Δ transformed with the empty vector (□) and with plasmids holding DNA fragments from the strain ISA1307 genomic library B02 (■), B18 (▲), L04 (♦), S06 (▼) and X25 (●) cloned in vector pRS316. S. cerevisiae BY4741 parental strain transformed with the empty vector (○) was included in the same 96-well microplate as a positive control. b S. cerevisiae BY4741 parental strain transformed with the empty vector (○) or with plasmids containing DNA fragments from the strain ISA1307 genomic library B02 (■), B18 (▲), S06 (▼), S07 (+) and W08 (×). The selected strains were those with a significant decreased duration of the latency period of the deletion mutant or in the parental strain when compared with the corresponding strain containing the empty vector. The growth curves were performed in MM4 medium (pH 4.0) without uracil, supplemented with 60 mM acetic acid (pH 4.0) and are representative of at least three independent growth assays that gave rise to similar growth curves