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Table 2 Genomic DNA inserts from strain ISA1307 genomic library containing the strong candidate determinants of tolerance to acetic acid proposed in this work

From: Search for genes responsible for the remarkably high acetic acid tolerance of a Zygosaccharomyces bailii-derived interspecies hybrid strain

DNA insert ORFa Identity (%) with Z. bailii CLIB213Tb S.cerevisiae putative homologue Identity (%) with S. cerevisiae c Size (aa) ISA1307 / S. cerevisiae / Alignmentd S.cerevisiae functione Alteration in yeast deletion mutant susceptibility to weak organic acidsf
8.23 (2782 bp) ZBAI_05695 99 GYP8 34.0 556 / 497 / 503 GTPase-activating protein involved in the regulation of ER to Golgi vesicle transportCellular transport and transport routes -
18.22 (2803 bp) ZBAI_04770 (41 %) 95/95 PMT1 66.9 761 / 817 / 746 Protein O-mannosyltransferase Acetic acid (S) [8]
Protein fate
23.1 (2277 bp) ZBAI_09707 (40 %) 92/95 WSC4 29.1 854 / 605 / 632 ER membrane protein involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response -
Cellular transport and transport routes
23.17 (1812 bp) ZBAI_09663 (66 %) 99/99 ILV3 82.5 583 / 585 / 584 Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids -
Amino acid metabolism
B02 (3640 bp) ZBAI_03527 (77 %) 95/95 MSN4 32.9 574 / 630 / 590 Transcriptional activator that regulates the general stress response of S. cerevisiae 2,4-D (S) [41]
Transcription
B18 (2917 bp) ZBAI_01028 94 TIF3 55.8 433 / 436 / 453 Translation initiation factor eIF-4B Acetic acid (S) [42]Propionic acid (S) [7]
Protein synthesis
S07 (2860 bp) ZBAI_05420 (57 %) 95/95 KTR7 49.3 514 / 517 / 525 Putative mannosyltransferase involved in protein glycosylation -
Protein fate
S06 (4413 bp) ZBAI_02295 99 - - 178 / - / - - -
ZBAI_02296 98 - - 261 / - / - - -
Y08 (2104 bp) ZBAI_01926 (34 %) 92/93 RKR1 48.8 1555 / 1562 / 1571 RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins -
Protein fate
  1. aPercentage of nucleotides present in the truncated ORFs, compared with the total ORF sequence, is indicated in parentheses
  2. bIdentity (%) between each ORF found in the DNA inserts and Z. bailii CLIB213T genome was obtained using BLAST analysis (http://blast.ncbi.nlm.nih.gov/Blast.cgi)
  3. cIdentity (%) between ISA1307 and S. cerevisiae S288C homologous genes was retrieved from PEDANT database (http://pedant.helmholtz-muenchen.de/genomes.jsp?Category=fungal)
  4. dIdentity (%) between homologous proteins from ISA1307 and S. cerevisiae. The size of the proteins and their pairwise alignment, given by the number of amino acid residues (aa), was retrieved from the PEDANT database (http://pedant.helmholtz-muenchen.de/genomes.jsp?Category=fungal)
  5. eThe putative function of each ORF was assigned based on the function of each S. cerevisiae homologous gene (www.yeastgenome.org). The functional category is provided in bold
  6. fList of the studies involving the S. cerevisiae corresponding deletion mutant susceptibility or resistance phenotypes under weak acid stress (S) - the single deletion mutant is susceptible to the acid; (R) - the single deletion mutant is resistant to the acid