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Fig. 12 | BMC Genomics

Fig. 12

From: Genome-wide analysis, molecular cloning and expression profiling reveal tissue-specifically expressed, feedback-regulated, stress-responsive and alternatively spliced novel genes involved in gibberellin metabolism in Salvia miltiorrhiza

Fig. 12

Alternative splicing and tissue-specific expression of various GA metabolism pathway genes in S. miltiorrhiza. a–d: Gene structures of SmKO (a), SmGA20ox3 (b), SmGA2ox3 (c), SmGA2ox11 (d) and their splice variants. Filled boxes represent exons. Lines represent introns. e–h: Fold changes of SmKOv1 (e), SmGA20ox3v (f), SmGA2ox3v (g) and SmGA2ox11v1 (h) in roots (Rt), stems (St), leaves (Le) and flowers (Fl) of S. miltiorrhiza plants. The expression levels were analyzed using the quantitative RT-PCR method. Expression level in leaves was arbitrarily set to 1 and the levels in other tissues were given relative to this. Error bars represent standard deviations of mean value from three biological and four technical replicates. ANOVA (analysis of variance) was calculated using SPSS. P < 0.05 was considered statistically significant

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