Genome-wide analysis, molecular cloning and expression profiling reveal tissue-specifically expressed, feedback-regulated, stress-responsive and alternatively spliced novel genes involved in gibberellin metabolism in Salvia miltiorrhiza

Table 1 Sequence features of GA metabolism pathway genes in S. miltiorrhiza

Gene name	Accession number	ORF^a (bp)	Len^b	MW^c (kDa)	pI	Loc^d
SmKO	KT853074	1557	519	58.73	7.63	S
SmKAO1	KT853075	1302	434	50.00	9.33	S
SmKAO2	KT853076	1431	477	54.82	9.43	S
SmGA3ox1	KT853077	1038	346	37.86	6.70	-
SmGA3ox2	KT853078	1092	364	39.92	7.74	-
SmGA20ox1	KT853079	1134	378	43.01	6.38	-
SmGA20ox2	KT853080	1173	391	43.83	6.56	-
SmGA20ox3	KT853081	1155	385	43.01	7.69	-
SmGA20ox4	KT853082	1176	392	44.09	6.11	C
SmGA20ox5	KT853083	1044	348	38.50	5.99	-
SmGA20ox6	KT853084	1050	350	39.99	5.84	-
SmGA2ox1	KT853085	1122	374	42.12	7.54	C
SmGA2ox2	KT853086	966	322	35.70	5.60	-
SmGA2ox3	KT853087	936	312	34.66	6.12	-
SmGA2ox4	KT853088	1005	335	37.67	6.22	-
SmGA2ox5	KT853089	1002	334	38.15	7.29	C
SmGA2ox6	KT853090	987	329	37.39	5.56	-
SmGA2ox7	KT853091	939	313	35.16	5.38	-
SmGA2ox8	KT853092	1005	335	37.19	7.08	-
SmGA2ox9	KT853093	933	311	34.36	5.85	-
SmGA2ox10	KT853094	1137	379	41.83	5.69	-
SmGA2ox11	KT853095	972	324	36.01	6.67	-

^aORF represents open reading frame
^bLen represents the number of amino acid residues
^cMW represents molecular weight
^dLoc represents the protein localization predicted by TargetP1.1. ‘S’ stands for secretory pathway, showing that the sequence cotains a signal peptide. ‘C’ stands for chloroplast, suggesting that the sequence contains a chloroplast transit peptide. ‘-’ indicates any locations other than the plastid, mitochondrion and secretory pathway

ISSN: 1471-2164