Fig. 7
Analysis of the transcriptional differences between 7G8pfcrt_CTL and 7G8pfcrt_T76K. a Genes showing higher expression (fold change >1.5 and normalized fold change ≥2 SD above the mean) in the recombinant control 7G8pfcrt_CTL in comparison to the recombinant back-mutant strain 7G8pfcrt_T76K. Gene expression fold changes were calculated as the difference between the areas under the curve generated on the full 384 time point data and were then normalized using a baseline created by the background pool of 11 transcriptomes. b Genes showing a lower expression (fold change <0.6 and normalized fold change ≤3 SD below the mean) in the recombinant control 7G8pfcrt_CTL in comparison to the recombinant back-mutant strain 7G8pfcrt_T76K . c HATS analysis was used to identify gene sets that are enriched in 7G8pfcrt_CTL in comparison to 7G8pfcrt_T76K (mean ranking values >0.9). Enrichment scores were normalized using the expression baseline constituted by the background pool of 11 transcriptome data sets. d Gene sets that are depleted in 7G8pfcrt_CTL in comparison to 7G8pfcrt_T76K (mean ranking values <0.1), as identified from HATS analysis. e Stage-associated enrichment analysis. Gene sets with significant enrichment (orange) or depletion (blue) in 7G8pfcrt_CTL in comparison to 7G8pfcrt_T76K for three windows of the parasite IDC corresponding to rings (R, 12–16 h post-invasion), trophozoites (T, 24–28 h post-invasion) and schizonts (S, 32–36 h post-invasion) using stage-enrichment analysis