Fig. 3

Direction and magnitude of natural selection acting on Cucurbitaceae clade V MLO homologs. a) Codon-based dN-dS values and sequence logo indicating nucleotide conservation along the alignment of Cucurbitaceae Clade V MLO coding sequences. Blue and red dots indicate positive and negative values, respectively. Green circles indicate protein regions containing codons predicted to be subjected to positive selection, and the star indicates a codon characterized by a significant p-value (p < 0.1) for positive selection (in position 252 of the alignment). Aligned sequences are also indicated, together with gene regions predicted to encode for extracellular, transmembrane and intracellular protein regions (indicated with blue, red and green bars, respectively). b) Close-up view on the gene region containing the codon in position 252 of the alignment