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Table 1 Putative SFPs identified in the LHT of Melanoplus sanguinipes

From: Combining RNA-seq and proteomic profiling to identify seminal fluid proteins in the migratory grasshopper Melanoplus sanguinipes (F)

Contig

Expression patterns

Functional summary

Reads

EmPAI

Genome matched (Reference)

BLASTP description

Orthology

Signal secretion

E-value

Hit Rank

Bit score

Proteolysis regulators

MS0199

a

Dipeptidase

58

6.07

A. fasciatus (2)

EG018903.1 Af_ARG_10E12_sp6 Accessory gland

6.00E-63

2

208

SP

MS1698

a

Serine protease inhibitor

60

1.06

H. melpomne (2)

HM023856.2| SFP HACP059

4.00E-45

1

139

SP

MS3398

a

Serpin b6

21

2.15

H. melpomene (2)

HM023856.2 SFP HACP059

9.00E-23

4

134

SP

MS1783

a

Angiotensin-converting enzyme

93

0.96

L. migratoria (3)

AY487174.1| Angiotensin converting enzyme

0

1

803

SP

MS0381

a

Metallo-endopeptidase (nepriyisin)

36

0.98

G. firmus (1)

EB409838.1 Gf_AcGl1_07A05_SP6 AcGl1

2.00E-34

1

127

SP

MS0081

a

Serine protease

481

6.21

A. fasciatus (1)

GQ911573.1 ejaculate serine protease (ejac-sp)

5.00E-80

1

168

NC

MS0089

a

Serine protease

481

28.8

H. melpomene (2)

HM023850.1 SFP HACP049

7.00E-61

1

119

NC

MS3857

a

Serine carboxypeptidase

6

1.5

Ae. Aegypti (6)

XM_001660689.1 Zinc carboxypeptidase

6.00E-48

1

116

NC

MS2174

b

Serine protease

29

2.17

H. melpomene (2)

HM023834.1 SFP HACP027

3.00E-36

1

148

NC

MS1693

a

Serine protease

175

0.29

H. melpomene (2)

HM023850.1 SFP

2.00E-21

1

40.5

SP

MS3819

b

Transmembrane protease serine

6

0.6

G. firmus (1)

DQ520137.1 Accessory gland protein (AG-0159F)

1.00E-10

4

46.4

SP

MS2043

a

Serine protease

8

0.75

G. firmus (1)

EB409712.1Gf_AcGl1_08D06_SP6

46.0E-10

4

45.5

SP

MS5974

b

Serine protease inhibitor

4

0.2

-

-

-

-

-

SP

MS0294

a

Serine carboxypeptidase

146

9.86

-

-

-

-

-

SP

MS6504

a

Serine carboxypeptidase

8

1.26

-

-

-

-

-

SP

Environmental stress proteins

MS0126

c

Heat shock protein

84

0.79

T. casteanum (5)

XM_965476.2 70kD protein cognate (LOC659147)

1.00E-155

1

538

SP

MS0131

b

Heat shock protein

17

1.59

T. casteanum (5)

XM_961518.2. 70kD protein cognate 70, LOC657158

0

1

1216

-

Iron storage proteins

MS1628

b

Transferrin

33

4.26

G. firmus (1)

EB409584.1 Gf_AcGl1_03G10_SP6 AcGl1

2.00E-69

1

256

SP

MS0380

b

Ferritin subunit

51

0.14

-

-

-

-

-

SP

Oxidative stress proteins

MS1782

b

Catalase

4

1

Ae. Aegypti (6)

XM_001663550.1 Aedes aegypti catalase partial

4.00E-115

1

290

SP

MS0023

b

Aldo-keto reductase

323

2.56

H. erato (2)

HM023814.1 Seminal fluid protein HACP058

2.00E-57

1

102

NC

MS2301

c

Phenoloxidase

26

1.69

T. casteanum (5)

NM_001039433.1| Tribolium castaneum pro-phenol oxidase subun…

0

1

286

NC

MS0678

c

Glutathione s-transferase

12

2.88

Ae. Aegypti (6)

XM_001661819.1 glutathione s-transferase

4.00E-55

1

203

-

MS0031

b

Endoplasmin

142

0.17

-

-

-

-

-

SP

MS4425

b

Aldehyde oxidase

7

0.3

-

-

-

-

-

SP

     

Lipases

     

MS5352

c

Lipase

10

4.85

Ae. Aegypti (6)

XM_001651399.1| vitellogenin partial

1.00E-24

1

109

SP

MS1414

a

Lipase

19

1.53

-

-

-

-

-

SP

MS0358

a

Lipase

119

21.37

-

-

-

-

-

SP

Cell adhesion protein, membrane-bound and structural proteins

MS2129

b

Moesin ezrin radixin

42

3.91

Ae. Aegypti (6)

XM_001652925. Moesin/ezrin/radixin

0

1

903

 

MS0111

b

Actin

446

2.56

G. firmus (1)

EB409566 Gf_AcGl1_03F03_SP6 5

1.00E-92

1

333

NC

MS0014

b

Annexin isoform a

85

0.11

T. casteanum (5)

XM_962838.2 PREDICTED: similar to Annexin IX CG5730-PC

9.00E-147

1

515

-

MS0238

b

Elongation factor 1-alpha

238

1.67

A. fasciatus (2)

EG018577.1 Af_ARG_09B03_sp6 Accessory gland

3.00E-127

1

449

-

MS0234

b

Elongation factor 2

864

0.46

G. firmus (1)

EB409600.1 Gf_AcGl1_01A02_SP6 AcGl1

2.00E-128

1

453

-

Others

MS0219

b

Calreticulin

343

0.37

-

-

-

-

-

SP

MS0105

b

Midline fasciclin

287

4.08

A. fasciatus (2)

EG018790.1 Af_ARG_02F01_sp6 Accessory gland

2.00 E-75

1

169

SP

MS0076

a

Hypothetical EAI_15996

6173

73.01

G. firmus (1)

EB409920.1 Gf_AcGl1_06A06_SP6

2.00E-47

1

184

SP

MS0157

b

Nucleoside diphosphate kinase

154

1.43

Ae. Aegypti (6)

XM_001662462.1Aedes aegypti nucleoside-diphosphate kinase

3.00E-87

1

310

SP

MS1368

b

Protein 5nuc-like

99

0.66

G. firmus (1)

EB409815.1 Gf_AcGl1_09F10_SP6

3.00E-30

1

110

SP

MS0047

b

Peptidyl-prolyl cis-trans isomerase 5

106

4.97

G. firmus (1)

EU669817.1 Peptidyl-prolyl isomerase-1

3.00E-45

2

174

SP

MS1669

c

Aminopeptidase -like

12

0.18

Ae. Aegypti (6)

XM_001656177.1 Aedes aegypti leucyl amino peptidase partial

2.00E-79

1

142

-

MS1026

b

Peptidoglycan recognition protein

23

2.22

-

-

-

-

-

SP

MS2092

b

Hypoxia up-regulated protein

12

0.73

-

-

-

-

-

SP

MS7155

a

Juvenile hormone-inducible

7

0.12

-

-

-

-

-

SP

MS0013

a

Oviposition Stimulating Protein (OSP)

28685

151323.3

-

-

-

-

-

SP

MS0682

b

Proactivator polypeptide

209

0.08

-

-

-

-

-

SP

MS0237

c

Carbonic anhydrase 2-like

18

1.56

-

-

-

-

-

SP

MS0221

c

Neutral alpha-glucosidase ab-like

24

0.45

-

-

-

-

-

SP

MS0644

b

Profilin

149

0.54

-

-

-

-

-

SP

MS2078

c

Plasma alpha-l-fucosidase

21

1.3

-

-

-

-

-

SP

MS0168

c

Esterase fe4-like

32

1.81

-

-

-

-

-

SP

MS0673

 

AaeL_AAEL001498

24

0.55

     

SP

MS3488

b

Hemomucin

10

1.07

-

-

-

-

-

SP

MS0097

b

Pdgf- and vegf-related factor 1-like precursor

6

0.26

-

-

-

-

-

SP

MS1006

b

Imaginal disc growth factor 4

75

4.26

-

-

-

-

-

SP

MS1117

c

Calumenin

19

0.23

-

-

-

-

-

SP

Unknown

MS0327

c

Unknown

164

1.54

-

-

-

-

-

SP

MS1333

a

Unknown

55

3.39

-

-

-

-

-

SP

MS0039

a

Unknown

681

1.43

-

-

-

-

-

SP

MS2957

b

Unknown

13

0.18

-

-

-

-

-

SP

MS0784

b

Unknown

7

3.09

-

-

-

-

-

SP

MS0278

c

Unknown

375

0.51

-

-

-

-

-

SP

MS0281

c

Unknown

167

0.44

-

-

-

-

-

SP

MS5071

b

Unknown

2

1.92

-

-

-

-

-

SP

MS1809

c

Unknown

33

1.71

-

-

-

-

-

SP

  1. References ((1) Andres et al. [14, 40]; (2) Walters and Harrison. [32] (3) Macours et al. [107]; (4) Findlay et al. [29]; (5) Xu et al. [24]; (6) Sirot et al. [58]). Expression patterns using qualitative RT-PCR [(a) Male expression exclusively; (b) Expression in both sexes and (c) Not amplified. Signal secretion [Signal peptide (SP), Non-canonical secretion (NP)